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Literature summary for 1.11.1.21 extracted from

  • Singh, R.; Wiseman, B.; Deemagarn, T.; Jha, V.; Switala, J.; Loewen, P.
    Comparative study of catalase-peroxidases (KatGs) (2008), Arch. Biochem. Biophys., 471, 207-214.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Escherichia coli
expressed in Escherichia coli Geobacillus stearothermophilus
expressed in Escherichia coli Rhodobacter capsulatus
expressed in Escherichia coli Mycobacterium tuberculosis
expressed in Escherichia coli Synechocystis sp.
expressed in Escherichia coli Archaeoglobus fulgidus
expressed in Escherichia coli Burkholderia pseudomallei

Inhibitors

Inhibitors Comment Organism Structure
azide highly sensitive Archaeoglobus fulgidus
azide highly sensitive Burkholderia pseudomallei
azide highly sensitive Escherichia coli
azide highly sensitive Geobacillus stearothermophilus
azide highly sensitive Mycobacterium tuberculosis
azide highly sensitive Rhodobacter capsulatus
azide highly sensitive Synechocystis sp.
cyanide highly sensitive Archaeoglobus fulgidus
cyanide highly sensitive Burkholderia pseudomallei
cyanide highly sensitive Escherichia coli
cyanide highly sensitive Geobacillus stearothermophilus
cyanide highly sensitive Mycobacterium tuberculosis
cyanide highly sensitive Rhodobacter capsulatus
cyanide highly sensitive Synechocystis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase reaction, at pH 4.3 and 25°C Synechocystis sp.
0.016
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase reaction, at pH 4.5 and 25°C Archaeoglobus fulgidus
0.016
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase reaction, at pH 5.0 and 25°C Rhodobacter capsulatus
0.024
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase reaction, at pH 4.3 and 25°C Escherichia coli
0.031
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase reaction, at pH 4.0 and 25°C Geobacillus stearothermophilus
0.06
-
H2O2 peroxidase reaction, at pH 4.3 and 25°C Escherichia coli
0.067
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase reaction, at pH 4.8 and 25°C Mycobacterium tuberculosis
0.095
-
H2O2 peroxidase reaction, at pH 4.5 and 25°C Archaeoglobus fulgidus
0.21
-
H2O2 peroxidase reaction, at pH 4.0 and 25°C Geobacillus stearothermophilus
0.3
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase reaction, at pH 4.5 and 25°C Burkholderia pseudomallei
0.36
-
H2O2 peroxidase reaction, at pH 4.8 and 25°C Mycobacterium tuberculosis
0.7
-
H2O2 peroxidase reaction, at pH 4.5 and 25°C Burkholderia pseudomallei
0.83
-
H2O2 peroxidase reaction, at pH 5.0 and 25°C Rhodobacter capsulatus
1
-
H2O2 peroxidase reaction, at pH 4.3 and 25°C Synechocystis sp.
2.4
-
H2O2 catalase reaction, at pH 7.0 and 37°C Mycobacterium tuberculosis
3.1
-
H2O2 catalase reaction, at pH 7.0 and 37°C Synechocystis sp.
3.7
-
H2O2 catalase reaction, at pH 7.0 and 37°C Geobacillus stearothermophilus
3.7
-
H2O2 catalase reaction, at pH 7.0 and 37°C Rhodobacter capsulatus
3.8
-
H2O2 catalase reaction, at pH 7.0 and 37°C Archaeoglobus fulgidus
4.2
-
H2O2 catalase reaction, at pH 7.0 and 37°C Escherichia coli
4.5
-
H2O2 catalase reaction, at pH 7.0 and 37°C Burkholderia pseudomallei
20
-
H2O2 catalase reaction, at pH 5.5-6.0 and 37°C Synechocystis sp.
30
-
H2O2 catalase reaction, at pH 5.5-6.0 and 37°C Rhodobacter capsulatus
32
-
H2O2 catalase reaction, at pH 5.5-6.0 and 37°C Archaeoglobus fulgidus
35
-
H2O2 catalase reaction, at pH 5.5-6.0 and 37°C Escherichia coli
56
-
H2O2 catalase reaction, at pH 5.5-6.0 and 37°C Burkholderia pseudomallei
90
-
H2O2 catalase reaction, at pH 5.5-6.0 and 37°C Geobacillus stearothermophilus
225
-
H2O2 catalase reaction, at pH 5.5-6.0 and 37°C Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
about 80000, SDS-PAGE Escherichia coli
80000
-
about 80000, SDS-PAGE Geobacillus stearothermophilus
80000
-
about 80000, SDS-PAGE Rhodobacter capsulatus
80000
-
about 80000, SDS-PAGE Mycobacterium tuberculosis
80000
-
about 80000, SDS-PAGE Synechocystis sp.
80000
-
about 80000, SDS-PAGE Archaeoglobus fulgidus
80000
-
about 80000, SDS-PAGE Burkholderia pseudomallei

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus
-
-
-
Burkholderia pseudomallei
-
-
-
Escherichia coli
-
-
-
Geobacillus stearothermophilus
-
-
-
Mycobacterium tuberculosis
-
-
-
Rhodobacter capsulatus
-
-
-
Synechocystis sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and DEAE-cellulose column chromatography Escherichia coli
ammonium sulfate precipitation and DEAE-cellulose column chromatography Geobacillus stearothermophilus
ammonium sulfate precipitation and DEAE-cellulose column chromatography Rhodobacter capsulatus
ammonium sulfate precipitation and DEAE-cellulose column chromatography Mycobacterium tuberculosis
ammonium sulfate precipitation and DEAE-cellulose column chromatography Synechocystis sp.
ammonium sulfate precipitation and DEAE-cellulose column chromatography Archaeoglobus fulgidus
ammonium sulfate precipitation and DEAE-cellulose column chromatography Burkholderia pseudomallei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1
-
peroxidase specific activity, using o-dianisidine as substrate, at pH 5.0 and 25°C Rhodobacter capsulatus
2
-
peroxidase specific activity, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 5.0 and 25°C Rhodobacter capsulatus
3.2
-
peroxidase specific activity, using o-dianisidine as substrate, at pH 4.5 and 25°C Archaeoglobus fulgidus
4.8
-
peroxidase specific activity, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 4.5 and 25°C Burkholderia pseudomallei
5.3
-
peroxidase specific activity, using o-dianisidine as substrate, at pH 4.0 and 25°C Geobacillus stearothermophilus
5.3
-
peroxidase specific activity, using o-dianisidine as substrate, at pH 4.5 and 25°C Burkholderia pseudomallei
5.5
-
peroxidase specific activity, using o-dianisidine as substrate, at pH 4.3 and 25°C Synechocystis sp.
5.9
-
peroxidase specific activity, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 4.0 and 25°C Geobacillus stearothermophilus
6.4
-
peroxidase specific activity, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 4.3 and 25°C Synechocystis sp.
8.3
-
peroxidase specific activity, using o-dianisidine as substrate, at pH 4.3 and 25°C Escherichia coli
8.8
-
peroxidase specific activity, using o-dianisidine as substrate, at pH 4.8 and 25°C Mycobacterium tuberculosis
10
-
peroxidase specific activity, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 4.8 and 25°C Mycobacterium tuberculosis
11
-
peroxidase specific activity, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 4.3 and 25°C Escherichia coli
12
-
peroxidase specific activity, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 4.5 and 25°C Archaeoglobus fulgidus
2200
-
catalase specific activity, at pH 7.0 and 37°C Escherichia coli
3120
-
catalase specific activity, at pH 7.0 and 37°C Geobacillus stearothermophilus
3630
-
catalase specific activity, at pH 7.0 and 37°C Burkholderia pseudomallei
4450
-
catalase specific activity, at pH 7.0 and 37°C Mycobacterium tuberculosis
4830
-
catalase specific activity, at pH 7.0 and 37°C Rhodobacter capsulatus
5280
-
catalase specific activity, at pH 7.0 and 37°C Archaeoglobus fulgidus
5420
-
catalase specific activity, at pH 7.0 and 37°C Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
Escherichia coli oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
Geobacillus stearothermophilus oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
Rhodobacter capsulatus oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
Mycobacterium tuberculosis oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
Synechocystis sp. oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
Archaeoglobus fulgidus oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
Burkholderia pseudomallei oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
?
H2O2
-
Escherichia coli O2 + H2O
-
?
H2O2
-
Geobacillus stearothermophilus O2 + H2O
-
?
H2O2
-
Rhodobacter capsulatus O2 + H2O
-
?
H2O2
-
Mycobacterium tuberculosis O2 + H2O
-
?
H2O2
-
Synechocystis sp. O2 + H2O
-
?
H2O2
-
Archaeoglobus fulgidus O2 + H2O
-
?
H2O2
-
Burkholderia pseudomallei O2 + H2O
-
?
o-dianisidine + H2O2
-
Escherichia coli oxidized o-dianisidine + H2O
-
?
o-dianisidine + H2O2
-
Geobacillus stearothermophilus oxidized o-dianisidine + H2O
-
?
o-dianisidine + H2O2
-
Rhodobacter capsulatus oxidized o-dianisidine + H2O
-
?
o-dianisidine + H2O2
-
Mycobacterium tuberculosis oxidized o-dianisidine + H2O
-
?
o-dianisidine + H2O2
-
Synechocystis sp. oxidized o-dianisidine + H2O
-
?
o-dianisidine + H2O2
-
Archaeoglobus fulgidus oxidized o-dianisidine + H2O
-
?
o-dianisidine + H2O2
-
Burkholderia pseudomallei oxidized o-dianisidine + H2O
-
?

Synonyms

Synonyms Comment Organism
KatG
-
Escherichia coli
KatG
-
Geobacillus stearothermophilus
KatG
-
Rhodobacter capsulatus
KatG
-
Mycobacterium tuberculosis
KatG
-
Synechocystis sp.
KatG
-
Archaeoglobus fulgidus
KatG
-
Burkholderia pseudomallei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
the enzyme is completely inactive after less than 1 min at 65°C Rhodobacter capsulatus
65
-
the enzyme is completely inactive after less than 1 min at 65°C Synechocystis sp.
65
-
the enzyme is completely inactive after less than 1 min at 65°C Burkholderia pseudomallei
65
-
the enzyme is completely inactive after less than 1.5 min at 65°C Escherichia coli
65
-
the enzyme is completely inactive after less than 1.5 min at 65°C Mycobacterium tuberculosis
65
-
the enzyme is completely inactive after more than 40 min at 65°C Geobacillus stearothermophilus
65
-
the enzyme is completely inactive after more than 40 min at 65°C Archaeoglobus fulgidus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.7
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase reaction, at pH 5.0 and 25°C Rhodobacter capsulatus
7.9
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase reaction, at pH 4.5 and 25°C Burkholderia pseudomallei
11
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase reaction, at pH 4.0 and 25°C Geobacillus stearothermophilus
13
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase reaction, at pH 4.3 and 25°C Synechocystis sp.
17
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase reaction, at pH 4.5 and 25°C Archaeoglobus fulgidus
19
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase reaction, at pH 4.8 and 25°C Mycobacterium tuberculosis
25
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase reaction, at pH 4.3 and 25°C Escherichia coli
2950
-
H2O2 catalase reaction, at pH 7.0 and 37°C Escherichia coli
4300
-
H2O2 catalase reaction, at pH 7.0 and 37°C Geobacillus stearothermophilus
4350
-
H2O2 catalase reaction, at pH 7.0 and 37°C Mycobacterium tuberculosis
4970
-
H2O2 catalase reaction, at pH 5.5-6.0 and 37°C Escherichia coli
5680
-
H2O2 catalase reaction, at pH 7.0 and 37°C Burkholderia pseudomallei
6640
-
H2O2 catalase reaction, at pH 7.0 and 37°C Rhodobacter capsulatus
7600
-
H2O2 catalase reaction, at pH 5.5-6.0 and 37°C Geobacillus stearothermophilus
7630
-
H2O2 catalase reaction, at pH 7.0 and 37°C Synechocystis sp.
7770
-
H2O2 catalase reaction, at pH 7.0 and 37°C Archaeoglobus fulgidus
8000
-
H2O2 catalase reaction, at pH 5.5-6.0 and 37°C Synechocystis sp.
10200
-
H2O2 catalase reaction, at pH 5.5-6.0 and 37°C Mycobacterium tuberculosis
14100
-
H2O2 catalase reaction, at pH 5.5-6.0 and 37°C Rhodobacter capsulatus
15680
-
H2O2 catalase reaction, at pH 5.5-6.0 and 37°C Archaeoglobus fulgidus
15900
-
H2O2 catalase reaction, at pH 5.5-6.0 and 37°C Burkholderia pseudomallei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
Geobacillus stearothermophilus
4.3
-
-
Escherichia coli
4.3
-
-
Synechocystis sp.
4.5
-
-
Archaeoglobus fulgidus
4.5
-
-
Burkholderia pseudomallei
4.8
-
-
Mycobacterium tuberculosis
5
-
-
Rhodobacter capsulatus

Cofactor

Cofactor Comment Organism Structure
heme
-
Escherichia coli
heme
-
Geobacillus stearothermophilus
heme
-
Rhodobacter capsulatus
heme
-
Mycobacterium tuberculosis
heme
-
Synechocystis sp.
heme
-
Archaeoglobus fulgidus
heme
-
Burkholderia pseudomallei

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0002
-
at pH 7.0 and 37°C Mycobacterium tuberculosis azide
0.002
-
at pH 7.0 and 37°C Geobacillus stearothermophilus azide
0.007
-
at pH 7.0 and 37°C Escherichia coli azide
0.008
-
at pH 7.0 and 37°C Rhodobacter capsulatus azide
0.008
-
at pH 7.0 and 37°C Burkholderia pseudomallei azide
0.013
-
at pH 7.0 and 37°C Synechocystis sp. azide
0.02
-
at pH 7.0 and 37°C Archaeoglobus fulgidus azide
0.04
-
at pH 7.0 and 37°C Burkholderia pseudomallei cyanide
0.05
-
at pH 7.0 and 37°C Escherichia coli cyanide
0.05
-
at pH 7.0 and 37°C Geobacillus stearothermophilus cyanide
0.08
-
at pH 7.0 and 37°C Rhodobacter capsulatus cyanide
0.1
-
at pH 7.0 and 37°C Mycobacterium tuberculosis cyanide
0.15
-
at pH 7.0 and 37°C Archaeoglobus fulgidus cyanide
0.17
-
at pH 7.0 and 37°C Synechocystis sp. cyanide