Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NaCl | 76% stimulation of catalase by addition of 10 mM NaCl | Oceanobacillus oncorhynchi subsp. incaldanensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | strong inhibition | Oceanobacillus oncorhynchi subsp. incaldanensis | |
Ba2+ | partial inhibition of catalase activity | Oceanobacillus oncorhynchi subsp. incaldanensis | |
Co2+ | partial inhibition of catalase activity | Oceanobacillus oncorhynchi subsp. incaldanensis | |
cyanide | - |
Oceanobacillus oncorhynchi subsp. incaldanensis | |
EDTA | 27% inhibition at 10 mM | Oceanobacillus oncorhynchi subsp. incaldanensis | |
H2O2 | 17% inhibition at 80 mM | Oceanobacillus oncorhynchi subsp. incaldanensis | |
histidine | 19% inhibition at 10 mM | Oceanobacillus oncorhynchi subsp. incaldanensis | |
hydroxylamine | strong inhibition | Oceanobacillus oncorhynchi subsp. incaldanensis | |
KCl | 50% inhibition at 20 mM | Oceanobacillus oncorhynchi subsp. incaldanensis | |
MgCl2 | 50% inhibition at 10 mM | Oceanobacillus oncorhynchi subsp. incaldanensis | |
additional information | the enzyme is insensitive to 10 mM 3-amino-1,2,4-triazole | Oceanobacillus oncorhynchi subsp. incaldanensis | |
NaCl | 50% inhibition at 5 mM | Oceanobacillus oncorhynchi subsp. incaldanensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
24 | - |
H2O2 | catalase activity, in 20 mM Tris-HCl buffer (pH 9.0), at 30°C | Oceanobacillus oncorhynchi subsp. incaldanensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Oceanobacillus oncorhynchi subsp. incaldanensis | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | iron is present at 1 atom per homodimer | Oceanobacillus oncorhynchi subsp. incaldanensis | |
K+ | 2 mM K+ have a stimulatory effect on catalase activity | Oceanobacillus oncorhynchi subsp. incaldanensis | |
Mg2+ | 2 mM Mg2+ have a stimulatory effect on catalase activity | Oceanobacillus oncorhynchi subsp. incaldanensis | |
Mn2+ | 2 mM Mn2+ have a stimulatory effect on catalase activity | Oceanobacillus oncorhynchi subsp. incaldanensis | |
Na+ | 2 mM Na+ have a stimulatory effect on catalase activity | Oceanobacillus oncorhynchi subsp. incaldanensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
4 * 72000, SDS-PAGE | Oceanobacillus oncorhynchi subsp. incaldanensis |
280000 | - |
gel filtration | Oceanobacillus oncorhynchi subsp. incaldanensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oceanobacillus oncorhynchi subsp. incaldanensis | - |
- |
- |
Oceanobacillus oncorhynchi subsp. incaldanensis 20AG | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography, Superdex 200 gel filtration, and Mono Q column chromatography | Oceanobacillus oncorhynchi subsp. incaldanensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
168 | - |
unpurified cytosolic fraction, peroxidase activity, in 50 mM Tris-HCl buffer (pH 8.5), at 30°C | Oceanobacillus oncorhynchi subsp. incaldanensis |
316 | - |
unpurified cytosolic fraction, catalase activity, in 20 mM Tris-HCl buffer (pH 9.0), at 30°C | Oceanobacillus oncorhynchi subsp. incaldanensis |
7512 | - |
after 44.7fold purification, peroxidase activity, in 50 mM Tris-HCl buffer (pH 8.5), at 30°C | Oceanobacillus oncorhynchi subsp. incaldanensis |
35000 | - |
after 110.7fold purification, catalase activity, in 20 mM Tris-HCl buffer (pH 9.0), at 30°C | Oceanobacillus oncorhynchi subsp. incaldanensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminoantipyrine + H2O2 | - |
Oceanobacillus oncorhynchi subsp. incaldanensis | oxidized 4-aminoantipyrine + H2O | - |
? | |
4-aminoantipyrine + H2O2 | - |
Oceanobacillus oncorhynchi subsp. incaldanensis 20AG | oxidized 4-aminoantipyrine + H2O | - |
? | |
H2O2 | - |
Oceanobacillus oncorhynchi subsp. incaldanensis | O2 + H2O | - |
? | |
H2O2 | - |
Oceanobacillus oncorhynchi subsp. incaldanensis 20AG | O2 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 72000, SDS-PAGE | Oceanobacillus oncorhynchi subsp. incaldanensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
for catalase activity | Oceanobacillus oncorhynchi subsp. incaldanensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | for catalase activity | Oceanobacillus oncorhynchi subsp. incaldanensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | the catalatic activity of the enzyme is stable for 24 h at 40°C. At 50°C the half life is about 18 h, while at 60°C over the first 4 h of incubation 50% of activity is observed | Oceanobacillus oncorhynchi subsp. incaldanensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
for catalase activity | Oceanobacillus oncorhynchi subsp. incaldanensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | for catalase activity | Oceanobacillus oncorhynchi subsp. incaldanensis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | the catalatic activity of the enzyme is stable in the pH range of 5.0 to 10.0 after 1 h of treatment at 40°C | Oceanobacillus oncorhynchi subsp. incaldanensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | the enzyme contains 1 heme per homodimer | Oceanobacillus oncorhynchi subsp. incaldanensis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
5 | - |
catalase activity, in 20 mM Tris-HCl buffer (pH 9.0), at 30°C | Oceanobacillus oncorhynchi subsp. incaldanensis | NaCl | |
10 | - |
catalase activity, in 20 mM Tris-HCl buffer (pH 9.0), at 30°C | Oceanobacillus oncorhynchi subsp. incaldanensis | MgCl2 | |
20 | - |
catalase activity, in 20 mM Tris-HCl buffer (pH 9.0), at 30°C | Oceanobacillus oncorhynchi subsp. incaldanensis | KCl |