General Stability | Organism |
---|---|
both the catalatic and the peroxidatic activities are similarly inactivated by exposure to photooxidative conditions. The enzyme is reduced by sodium dithionite | Rhodobacter capsulatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | - |
Rhodobacter capsulatus | |
cyanide | bo th enzymic activities of catalase-peroxidase are equally inhibited by cyanide | Rhodobacter capsulatus | |
H2O2 | both the catalatic and the peroxidatic activities are similarly inactivated by treatment with 1 mM H2O2 | Rhodobacter capsulatus | |
hydroxylamine | - |
Rhodobacter capsulatus | |
additional information | p-chloromercuribenzoate at concentrations up to 100 mM does not inhibit the enzyme | Rhodobacter capsulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
236000 | - |
gel filtration | Rhodobacter capsulatus |
Organic Solvent | Comment | Organism |
---|---|---|
chloroform | both the catalatic and the peroxidatic activities are similarly inactivated by exposure to chloroform | Rhodobacter capsulatus |
Ethanol | both the catalatic and the peroxidatic activities are similarly inactivated by exposure to ethanol | Rhodobacter capsulatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodobacter capsulatus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diaminobenzidine + H2O2 | - |
Rhodobacter capsulatus | oxidized diaminobenzidine + H2O | - |
? | |
H2O2 | - |
Rhodobacter capsulatus | O2 + H2O | - |
? | |
additional information | no reaction is detected with guaiacol or with ascorbate | Rhodobacter capsulatus | ? | - |
? | |
o-dianisidine + H2O2 | - |
Rhodobacter capsulatus | oxidized o-dianisidine + H2O | - |
? | |
pyrogallol + H2O2 | - |
Rhodobacter capsulatus | ? + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | - |
Rhodobacter capsulatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
both the catalatic and the peroxidatic activities are similarly inactivated by heating to 50°C | Rhodobacter capsulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 5.3 | peroxidatic activity | Rhodobacter capsulatus |
6 | 6.5 | catalatic activity | Rhodobacter capsulatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | there are 1-1.2 molecules of protoheme IX per tetrameric molecule | Rhodobacter capsulatus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rhodobacter capsulatus | isoelectric focusing | - |
4.5 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00215 | - |
catalatic activity, in 100 mM potassium phosphate, pH 6.3, at 30°C | Rhodobacter capsulatus | hydroxylamine | |
0.022 | - |
catalatic activity, in 100 mM potassium phosphate, pH 6.3, at 30°C | Rhodobacter capsulatus | cyanide | |
0.023 | - |
peroxidatic activity, in 100 mM potassium phosphate, pH 5.2, at 30°C | Rhodobacter capsulatus | cyanide | |
0.078 | - |
peroxidatic activity, in 100 mM potassium phosphate, pH 5.2, at 30°C | Rhodobacter capsulatus | hydroxylamine | |
0.15 | - |
catalatic activity, in 100 mM potassium phosphate, pH 6.3, at 30°C | Rhodobacter capsulatus | azide | |
0.73 | - |
peroxidatic activity, in 100 mM potassium phosphate, pH 5.2, at 30°C | Rhodobacter capsulatus | azide |