Any feedback?
Literature summary for 1.11.1.21 extracted from
- Purification and characterization of an intracellular catalase-peroxidase from Penicillium simplicissimum (1996), Eur. J. Biochem., 235, 192-198.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| azide | - |
Penicillium simplicissimum |  |
| cyanide | - |
Penicillium simplicissimum | |
| additional information | incubation for 60 min with 20 mM 3-amino-l,2,4-triazole has no effect on enzyme activity | Penicillium simplicissimum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10.8 | - |
H2O2 | catalase activity, at pH 6.4 and 25°C | Penicillium simplicissimum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| protoplast | - |
Penicillium simplicissimum | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 83000 | - |
2 * 83000, SDS-PAGE | Penicillium simplicissimum |
| 170000 | - |
gel filtration | Penicillium simplicissimum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium simplicissimum | - |
- |
- |
| Penicillium simplicissimum CBS 170.90 | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | PCP does not contain a significant amount of carbohydrate | Penicillium simplicissimum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, phenyl-Sepharose column chromatography, Q-Sepharose column chromatography, hydroxyapatite column chromatography, and Superdex PG-200 gel filtration | Penicillium simplicissimum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:veratryl alcohol-grown cell | - |
Penicillium simplicissimum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
peroxidase activity using guaicol as substrate, at pH 5.4 and 25°C | Penicillium simplicissimum |
| 16 | - |
peroxidase activity using 3,3'-diaminobenzidine as substrate, at pH 5.4 and 25°C | Penicillium simplicissimum |
| 28 | - |
peroxidase activity using 3,3'-dimethoxybenzidine as substrate, at pH 5.4 and 25°C | Penicillium simplicissimum |
| 77 | - |
peroxidase activity using 2,6-dimethoxyphenol as substrate, at pH 5.4 and 25°C | Penicillium simplicissimum |
| 150 | - |
peroxidase activity using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 5.4 and 25°C | Penicillium simplicissimum |
| 250 | - |
cell extract, at 25°C, pH 6.4 | Penicillium simplicissimum |
| 2960 | - |
purified enzyme, at 25°C, pH 6.4 | Penicillium simplicissimum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Penicillium simplicissimum | oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O | - |
? | |
| 2,6-dimethoxyphenol + H2O2 | - |
Penicillium simplicissimum | oxidized 2,6-dimethoxyphenol + H2O | - |
? | |
| 3,3'-diaminobenzidine + H2O2 | - |
Penicillium simplicissimum | oxidized 3,3'-diaminobenzidine + H2O | - |
? | |
| 3,3'-dimethoxybenzidine + H2O2 | - |
Penicillium simplicissimum | oxidized 3,3'-dimethoxybenzidine + H2O | - |
? | |
| guaiacol + H2O2 | - |
Penicillium simplicissimum | tetraguaiacol + H2O | - |
? | |
| H2O2 | - |
Penicillium simplicissimum | O2 + H2O | - |
? | |
| additional information | NADPH, vanilly1 alcohol and veratryl alcohol do not serve as electron donors | Penicillium simplicissimum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 83000, SDS-PAGE | Penicillium simplicissimum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PCP | - |
Penicillium simplicissimum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
the enzyme remains fully active at pH 5.0 for more than 15 min at 25°C | Penicillium simplicissimum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.4 | - |
pH optimum for peroxidase activity | Penicillium simplicissimum |
| 6.4 | - |
pH optimum for catalase activity | Penicillium simplicissimum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | at pH 5.0 all activity is lost within 5 min whereas no inactivation is observed between pH 7.0 and pH 9.0 at this temperature after a 10 h incubation | Penicillium simplicissimum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Penicillium simplicissimum | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
at 25°C, pH 6.4 | Penicillium simplicissimum | cyanide | |
| 0.017 | - |
at 25°C, pH 6.4 | Penicillium simplicissimum | azide | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
