Application | Comment | Organism |
---|---|---|
additional information | the enzyme can be used as biocatalyst for flavor production | Pleurotus sapidus |
Cloned (Comment) | Organism |
---|---|
expressed in Komagataella pfaffii GS115 cells | Pleurotus sapidus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | an increase of the hydrogen peroxide concentration of more than 0.1 mM leads to a continuous activity decrease | Pleurotus sapidus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | at pH 4.0 and 30°C | Pleurotus sapidus | |
1.025 | - |
Mn2+ | at pH 3.5 and 30°C | Pleurotus sapidus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | highest activity in the presence of 25 mM Mn2+. The enzyme activity rises with increasing Mn2+ concentration, but is not completely dependent on the addition of Mn2+ | Pleurotus sapidus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pleurotus sapidus | A0A6H0MBW1 | - |
- |
Purification (Comment) | Organism |
---|---|
HiTrap SP Sepharose column chromatography | Pleurotus sapidus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Pleurotus sapidus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-methyl isoeugenol + H2O2 + H+ | - |
Pleurotus sapidus | oxidized (E)-methyl isoeugenol + H2O | - |
? | |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+ | - |
Pleurotus sapidus | oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O | - |
? | |
alpha-methylstyrene + H2O2 + H+ | - |
Pleurotus sapidus | oxidized alpha-methylstyrene + H2O | - |
? | |
annatto + H2O2 + H+ | - |
Pleurotus sapidus | oxidized annatto + H2O | - |
? | |
beta-carotene + H2O2 + H+ | - |
Pleurotus sapidus | oxidized beta-carotene + H2O | - |
? | |
Mn2+ + H2O2 + H+ | - |
Pleurotus sapidus | Mn3+ + 2 H2O | - |
? | |
additional information | the enzyme lacks the typical activity towards anthraquinone dyes like Reactive Blue 19 and Reactive Black 5 | Pleurotus sapidus | ? | - |
- |
|
trans-anethole + H2O2 + H+ | - |
Pleurotus sapidus | 4-anisaldehyde + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 61000, recombinant enzyme, SDS-PAGE | Pleurotus sapidus |
? | x * 45000, native enzyme, SDS-PAGE | Pleurotus sapidus |
? | x * 54900, SDS-PAGE, recombinant enzyme, calculated from amino acid sequence | Pleurotus sapidus |
Synonyms | Comment | Organism |
---|---|---|
dye-decolorizing peroxidase | - |
Pleurotus sapidus |
DyP | - |
Pleurotus sapidus |
DyP-type peroxidase | - |
Pleurotus sapidus |
POX | - |
Pleurotus sapidus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Pleurotus sapidus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | the enzyme is relatively stable at temperatures from 20 to 60°C with a residual activity of more than 80%. At higher temperatures, a high loss of activity is observed due to protein denaturation, resulting in residual activities of less than 5% | Pleurotus sapidus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.8 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | at pH 4.0 and 30°C | Pleurotus sapidus | |
7.2 | - |
Mn2+ | at pH 3.5 and 30°C | Pleurotus sapidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
- |
Pleurotus sapidus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 5 | more than 50% of activity is conserved between pH 3.0 and 5.0. At lower or higher pH values, less than 25% of activity remains | Pleurotus sapidus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | 5.5 | the enzyme activity is more than 90% between pH 2.0 and 5.5 after 1 h of incubation | Pleurotus sapidus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Pleurotus sapidus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pleurotus sapidus | isoelectric focusing | - |
6.7 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7 | - |
Mn2+ | at pH 3.5 and 30°C | Pleurotus sapidus | |
184 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | at pH 4.0 and 30°C | Pleurotus sapidus |