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Literature summary for 1.11.1.19 extracted from

  • Li, F.; Shao, R.; Mao, Y.; Yu, W.; Yu, H.
    Enzyme cascade reaction involving lytic polysaccharide monooxygenase and dye-decolorizing peroxidase for chitosan functionalization (2021), J. Agric. Food Chem., 69, 1049-1056 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain X33 and in Escherichia coli Rosetta (DE3) cells Pleurotus ostreatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03246
-
H2O2 at pH 6.0 and 28°C Pleurotus ostreatus
0.1128
-
gallic acid at pH 6.0 and 28°C Pleurotus ostreatus

Organism

Organism UniProt Comment Textmining
Pleurotus ostreatus
-
-
-
Pleurotus ostreatus HAUCC 162
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+
-
Pleurotus ostreatus oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O
-
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+
-
Pleurotus ostreatus HAUCC 162 oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O
-
?
gallic acid + H2O2 + H+
-
Pleurotus ostreatus oxidized gallic acid + H2O
-
?
gallic acid + H2O2 + H+
-
Pleurotus ostreatus HAUCC 162 oxidized gallic acid + H2O
-
?

Synonyms

Synonyms Comment Organism
dye-decolorizing peroxidase
-
Pleurotus ostreatus
DyP
-
Pleurotus ostreatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
15.22
-
gallic acid at pH 6.0 and 28°C Pleurotus ostreatus
118.43
-
H2O2 at pH 6.0 and 28°C Pleurotus ostreatus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
130
-
gallic acid at pH 6.0 and 28°C Pleurotus ostreatus
3650
-
H2O2 at pH 6.0 and 28°C Pleurotus ostreatus