Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | - |
Streptomyces avermitilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.049 | - |
Acid Blue 324 | at pH 4.5 and 25°C | Streptomyces avermitilis | |
0.61 | - |
2,6-dimethoxyphenol | at pH 4.0 and 25°C | Streptomyces avermitilis | |
1.2 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | at pH 4.0 and 25°C | Streptomyces avermitilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces avermitilis | - |
- |
- |
Streptomyces avermitilis ATCC31267 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography and Superdex 75pg gel filtration | Streptomyces avermitilis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
aerial mycelium | - |
Streptomyces avermitilis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+ | - |
Streptomyces avermitilis | oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O | - |
? | |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+ | - |
Streptomyces avermitilis ATCC31267 | oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O | - |
? | |
2,6-dimethoxyphenol + H2O2 + H+ | - |
Streptomyces avermitilis | oxidized 2,6-dimethoxyphenol + H2O | - |
? | |
2,6-dimethoxyphenol + H2O2 + H+ | - |
Streptomyces avermitilis ATCC31267 | oxidized 2,6-dimethoxyphenol + H2O | - |
? | |
Acid Blue 324 + H2O2 + H+ | - |
Streptomyces avermitilis | oxidized Acid Blue 324 + H2O | - |
? | |
Acid Blue 324 + H2O2 + H+ | - |
Streptomyces avermitilis ATCC31267 | oxidized Acid Blue 324 + H2O | - |
? | |
additional information | no activity with AQ-2, M303, Reactive Black 5, and Reactive Red 33 | Streptomyces avermitilis | ? | - |
- |
|
additional information | no activity with AQ-2, M303, Reactive Black 5, and Reactive Red 33 | Streptomyces avermitilis ATCC31267 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, His6-tagged enzyme, SDS-PAGE | Streptomyces avermitilis |
? | x * 49400, His6-tagged enzyme, calculated from amino acid sequence | Streptomyces avermitilis |
Synonyms | Comment | Organism |
---|---|---|
dye-decolorizing peroxidase | - |
Streptomyces avermitilis |
DyP1 | - |
Streptomyces avermitilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | the enzyme retains almost all of its initial activity against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,6-dimethoxyphenol after incubation at 30°C or 40°C for 2 h. The peroxidase activity of the enzyme against ABTS significantly decreases after incubating at 50°C for 1 h and is almost completely lost following incubation at 60°C for 30 min. On the other hand, the activity of the enzyme against 2,6-dimethoxyphenol is maintained at 50°C | Streptomyces avermitilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
2,6-dimethoxyphenol | at pH 4.0 and 25°C | Streptomyces avermitilis | |
0.79 | - |
Acid Blue 324 | at pH 4.5 and 25°C | Streptomyces avermitilis | |
2.8 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | at pH 4.0 and 25°C | Streptomyces avermitilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
the enzyme shows the highest activity for 2,6-dimethoxyphenol at pH 4.0 | Streptomyces avermitilis |
4.5 | - |
the enzyme shows the highest activity for 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) or Acid Blue 324 at pH 4.5 | Streptomyces avermitilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Streptomyces avermitilis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
13 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | at pH 4.0 and 25°C | Streptomyces avermitilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
2,6-dimethoxyphenol | at pH 4.0 and 25°C | Streptomyces avermitilis | |
2.4 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | at pH 4.0 and 25°C | Streptomyces avermitilis | |
16 | - |
Acid Blue 324 | at pH 4.5 and 25°C | Streptomyces avermitilis |