Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta(DE3)pLysS cells | Dictyostelium discoideum |
Crystallization (Comment) | Organism |
---|---|
enzyme in the absence of substrate as well as in the presence of potassium cyanide and veratryl alcohol, hanging drop vapor diffusion method, using 2.4 M sodium malonate pH 7.0 | Dictyostelium discoideum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
Reactive Blue 4 | at pH 3.0 and 25°C | Dictyostelium discoideum | |
0.166 | - |
veratryl alcohol | at pH 4.0 and 25°C | Dictyostelium discoideum | |
0.37 | - |
H2O2 | at pH 4.0 and 25°C | Dictyostelium discoideum | |
4.1 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | at pH 4.0 and 25°C | Dictyostelium discoideum | |
119 | - |
pyrogallol | at pH 4.0 and 25°C | Dictyostelium discoideum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyostelium discoideum | Q556V8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+ | - |
Dictyostelium discoideum | oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O | - |
? | |
guaiacylglycerol-beta-guaiacyl ether + H2O2 + H+ | - |
Dictyostelium discoideum | oxidized guaiacylglycerol-beta-guaiacyl ether + H2O | - |
? | |
pyrogallol + H2O2 + H+ | - |
Dictyostelium discoideum | oxidized pyrogallol + H2O | - |
? | |
Reactive Blue 4 + H2O2 + H+ | - |
Dictyostelium discoideum | oxidized Reactive Blue 4 + H2O | - |
? | |
veratryl alcohol + H2O2 + H+ | - |
Dictyostelium discoideum | veratraldehyde + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Dictyostelium discoideum |
Synonyms | Comment | Organism |
---|---|---|
dye-decolorizing peroxidase | - |
Dictyostelium discoideum |
DyPA | - |
Dictyostelium discoideum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 40 | the enzyme remains stable from 20-40°C | Dictyostelium discoideum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000338 | - |
veratryl alcohol | at pH 4.0 and 25°C | Dictyostelium discoideum | |
5.2 | - |
Reactive Blue 4 | at pH 3.0 and 25°C | Dictyostelium discoideum | |
7.9 | - |
pyrogallol | at pH 4.0 and 25°C | Dictyostelium discoideum | |
89.9 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | at pH 4.0 and 25°C | Dictyostelium discoideum | |
93.65 | - |
H2O2 | at pH 4.0 and 25°C | Dictyostelium discoideum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
optimum pH for oxidation of Reactive Blue 4 | Dictyostelium discoideum |
4 | - |
optimum pH for oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), pyrogallol and veratryl alcohol | Dictyostelium discoideum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Dictyostelium discoideum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00203 | - |
veratryl alcohol | at pH 4.0 and 25°C | Dictyostelium discoideum | |
0.06638 | - |
pyrogallol | at pH 4.0 and 25°C | Dictyostelium discoideum | |
21.9 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | at pH 4.0 and 25°C | Dictyostelium discoideum | |
130 | - |
Reactive Blue 4 | at pH 3.0 and 25°C | Dictyostelium discoideum | |
253 | - |
H2O2 | at pH 4.0 and 25°C | Dictyostelium discoideum |