Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Pseudomonas fluorescens |
General Stability | Organism |
---|---|
after five cycles, the Ca-alginate-immobilized enzyme system retains more than 58% of its initial activity | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Pseudomonas fluorescens Pf-5 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+ | - |
Pseudomonas fluorescens | oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O | - |
? | |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+ | - |
Pseudomonas fluorescens Pf-5 | oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O | - |
? | |
Reactive Blue 5 + H2O2 + H+ | the immobilized enzyme is able to decolorize Reactive Blue 5 at concentrations of 0.1, 0.05, and 0.01% (w/v) with efficiency rates of about 20, 29, and 45%, respectively | Pseudomonas fluorescens | oxidized Reactive Blue 5 + H2O | - |
? | |
Reactive Blue 5 + H2O2 + H+ | the immobilized enzyme is able to decolorize Reactive Blue 5 at concentrations of 0.1, 0.05, and 0.01% (w/v) with efficiency rates of about 20, 29, and 45%, respectively | Pseudomonas fluorescens Pf-5 | oxidized Reactive Blue 5 + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dye decolorizing peroxidases type B | - |
Pseudomonas fluorescens |
DyP1B | - |
Pseudomonas fluorescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
temperature optimum for free enzyme | Pseudomonas fluorescens |
40 | - |
temperature optimum for enzyme immobilized in Ca-alginate ferromagnetic beads | Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
pH optimum for free enzyme | Pseudomonas fluorescens |
7 | - |
pH optimum for enzyme immobilized in Ca-alginate ferromagnetic beads | Pseudomonas fluorescens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | free enzyme shows 25% of initial activity within a pH range between 6.0 and 8.0. The immobilized enzyme displays better activity in a broader pH range than the free peroxidase, which reaches around 58, 72, 82, 88, and 71% at pH 5.5, 6.0, 6.5, 7.5, and 8.0, respectively | Pseudomonas fluorescens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | the immobilized enzyme has an optimum stability at pH ranging from 5.5 to 6.0 compared with pH ranging from 4.0 to 5.0 for the free peroxidase. In these pH ranges, both enzymes show 50% activity after 72 h incubation | Pseudomonas fluorescens |