Literature summary for 1.11.1.19 extracted from

Dhankhar, P.; Dalal, V.; Mahto, J.K.; Gurjar, B.R.; Tomar, S.; Sharma, A.K.; Kumar, P.
Characterization of dye-decolorizing peroxidase from Bacillus subtilis (2020), Arch. Biochem. Biophys., 693, 108590 .

Search for more literature for 1.11.1.19

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment)	Organism
HEPES-bound enzyme, sitting drop vapor diffusion method, using 25% (v/v) 2-methyl-2,4-pentanediol, 50 mM HEPES, pH 7.0	Bacillus subtilis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0424	-	Reactive Black 5	at pH 4.0 and 25°C	Bacillus subtilis
0.0442	-	Methyl Violet	at pH 4.0 and 25°C	Bacillus subtilis
0.072	-	malachite green	at pH 4.0 and 25°C	Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	P39597	-	-
Bacillus subtilis 168	P39597	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA agarose column chromatography	Bacillus subtilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Malachite Green + H2O2 + H+	73.63% decolorization after 30 min	Bacillus subtilis	oxidized Malachite Green + H2O	-	?
Malachite Green + H2O2 + H+	73.63% decolorization after 30 min	Bacillus subtilis 168	oxidized Malachite Green + H2O	-	?
Methyl Violet + H2O2 + H+	63.89% decolorization after 30 min	Bacillus subtilis	oxidized Methyl Violet + H2O	-	?
Methyl Violet + H2O2 + H+	63.89% decolorization after 30 min	Bacillus subtilis 168	oxidized Methyl Violet + H2O	-	?
Reactive Black 5 + H2O2 + H+	32.96% decolorization after 30 min	Bacillus subtilis	oxidized Reactive Black 5 + H2O	-	?
Reactive Black 5 + H2O2 + H+	32.96% decolorization after 30 min	Bacillus subtilis 168	oxidized Reactive Black 5 + H2O	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 41000, SDS-PAGE	Bacillus subtilis

Synonyms

Synonyms	Comment	Organism
dye-decolorizing peroxidase	-	Bacillus subtilis
DyP	-	Bacillus subtilis

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
4	40	the enzyme is highly active (more than 90% activity) between 4 and 30°C and shows about 60% and 30% activity at 40°C and 50°C, respectively	Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.015	-	Reactive Black 5	at pH 4.0 and 25°C	Bacillus subtilis
0.022	-	Methyl Violet	at pH 4.0 and 25°C	Bacillus subtilis
0.0456	-	malachite green	at pH 4.0 and 25°C	Bacillus subtilis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3	-	pH optimum for reaction with Reactive Black 5	Bacillus subtilis
4	-	pH optimum for reaction with Malchite Green and Methyl Violet	Bacillus subtilis

pH Range

pH Minimum	pH Maximum	Comment	Organism
2	5	the enzyme retains over 60-70% of the peak activity at pH 3.0 and 5.0 for both Malchite Green and Methyl Violet. The enzyme retains more than 60% of its activity at pH 2.0 and 4.0 for Reactive Black 5	Bacillus subtilis

Cofactor

Cofactor	Comment	Organism	Structure
heme	-	Bacillus subtilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.36	-	Reactive Black 5	at pH 4.0 and 25°C	Bacillus subtilis
0.5	-	Methyl Violet	at pH 4.0 and 25°C	Bacillus subtilis
0.63	-	malachite green	at pH 4.0 and 25°C	Bacillus subtilis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)