Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
H2O2 | pH 4, 25°C | Bacillus subtilis | |
0.157 | - |
Reactive Blue 5 | pH 4, 25°C | Bacillus subtilis | |
0.166 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 4, 25°C | Bacillus subtilis | |
0.385 | - |
Mordant black 9 | pH 4, 25°C | Bacillus subtilis | |
0.444 | - |
Acid Blue 62 | pH 4, 25°C | Bacillus subtilis | |
0.499 | - |
guaiacol | pH 4, 25°C | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
gel filtration | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 | - |
Bacillus subtilis | ? + H2O | - |
? | |
Acid Blue 62 + H2O2 + H+ | - |
Bacillus subtilis | oxidized Acid Blue 62 + H2O | - |
? | |
guaiacol + H2O2 + H+ | - |
Bacillus subtilis | oxidized guaiacol + H2O | - |
? | |
Mordant Black 9 + H2O2 + H+ | - |
Bacillus subtilis | oxidized Mordant Black 9 + H2O | - |
? | |
additional information | poor activity with Mn2+, no substrates: Fe2+, syringaldehyde, acettosyringone, veratryl alcohol | Bacillus subtilis | ? | - |
? | |
Reactive Blue 5 + H2O2 + H+ | - |
Bacillus subtilis | oxidized Reactive Blue 5 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 48000, SDS-PAGE | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 30 | - |
Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | enzyme shows the typical Soret band, heme content is 0.5 mol per mol of protein | Bacillus subtilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
guaiacol | pH 4, 25°C | Bacillus subtilis | |
10 | - |
Mordant black 9 | pH 4, 25°C | Bacillus subtilis | |
20 | - |
Acid Blue 62 | pH 4, 25°C | Bacillus subtilis | |
50 | - |
Reactive Blue 5 | pH 4, 25°C | Bacillus subtilis | |
2000 | - |
H2O2 | pH 4, 25°C | Bacillus subtilis |