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Literature summary for 1.11.1.19 extracted from
- Decolorization of an anthraquinone dye by the recombinant dye-decolorizing peroxidase (rDyP) immobilized on mesoporous materials (2008), J. Mol. Catal. B, 54, 42-49.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Aspergillus oryzae strain RD005 | Thanatephorus cucumeris |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
gel filtration | Thanatephorus cucumeris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thanatephorus cucumeris | - |
strain Dec 1, formerly Geotrichum candidum Dec 1 | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| immobilized recombinant DyP has higher stability to H2O2 compared to free recombinant DyP. The stability of recombinant DyP immobilized on FSM-16 at pH 4.0 and on AlSBA-15 at pH 3.0 and 4 are similar to that of free recombinant DyP | Thanatephorus cucumeris |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation and butyl-Toyopearl column chromatography | Thanatephorus cucumeris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Reactive Blue 5 + H2O2 + H+ | - |
Thanatephorus cucumeris | oxidized Reactive Blue 5 + H2O | - |
? |  |
| Remazol Brilliant Blue R + H2O2 + H+ | - |
Thanatephorus cucumeris | oxidized Remazol Brilliant Blue R + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dye-decolorizing peroxidase | - |
Thanatephorus cucumeris |
| DyP | - |
Thanatephorus cucumeris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
overall efficiency is maximum for immobilized recombinant DyP on AlSBA-15 at pH 4.0 | Thanatephorus cucumeris |
| 5 | - |
overall efficiency is maximum for immobilized recombinant DyP on FSM-16 at pH 5.0 | Thanatephorus cucumeris |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6 | more than 90% of initial free recombinant DyP activity remains active after incubation in citrate buffered solution at pH 3.0-5.0 for 48 h. Adsorption yields of recombinant DyP immobilized on FSM-16 and AlSBA-15 increases as pH decreased from 6.0 to 3.0, however, the activity yield of immobilized recombinant DyP decreases with decreasing pH | Thanatephorus cucumeris |
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