Literature summary for 1.11.1.19 extracted from

Ogola, H.J.; Kamiike, T.; Hashimoto, N.; Ashida, H.; Ishikawa, T.; Shibata, H.; Sawa, Y.
Molecular characterization of a novel DyP-type peroxidase from the cyanobacterium Anabaena sp. PCC 7120 (2009), Appl. Environ. Microbiol., 75, 7509-7518.

Search for more literature for 1.11.1.19

Activating Compound

Activating Compound	Comment	Organism	Structure
syringaldehyde	the presence of syringaldehyde drastically enhances the rate of decolorization of Reactive Black 5, Reactive Orange 14, Reactive Red 120, Reactive Green 19, and Acid Red with 50fold, 9fold, 15fold, 2fold, and 7fold improvements, respectively	Anabaena sp.

Application

Application	Comment	Organism
additional information	AnaPX is a useful alternative of horseradish peroxidase or fungal DyPs	Anabaena sp.

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Anabaena sp.

Inhibitors

Inhibitors	Comment	Organism
diethyl dicarbonate	less than 50% inhibition at 50 mM	Anabaena sp.
Fe2+	AnaPX is highly sensitive to Fe2+ (98.8% inhibition at 5 mM)	Anabaena sp.
KCN	AnaPX shows very low sensitivity to KCN which causes only 9% and 14% inhibition of the activity at 1 and 10 mM, respectively	Anabaena sp.
Mn2+	57.1% inhibition at 5 mM	Anabaena sp.
additional information	the enzyme is not sensitive to the prototypical catalase inhibitor 3-amino-1,2,4,-triazole in the presence of ascorbic acid (1.0 mM), metal chelating and sulfhydryl reagents do not significantly affect activity	Anabaena sp.
NaN3	AnaPX is highly sensitive to NaN3 (82% inhibition at 10 mM)	Anabaena sp.
phenylhydrazine	AnaPX is highly sensitive to the suicide substrate phenylhydrazine (complete inhibition at 1 mM)	Anabaena sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0036	-	Reactive Blue 5	in 50 mM citrate buffer (pH 4.0-4.4), at 37°C	Anabaena sp.
0.0058	-	H2O2	in 50 mM citrate buffer (pH 4.0-4.4), at 37°C	Anabaena sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
53280	-	4 * 53280, native enzyme, MALDI-TOF spectrometry	Anabaena sp.
53368	-	4 * 53368, deduced from amino acid sequence	Anabaena sp.
54000	-	4 * 54000, native enzyme, SDS-PAGE	Anabaena sp.
209000	-	native enzyme, gel filtration	Anabaena sp.

Organism

Organism	UniProt	Comment	Textmining
Anabaena sp.	Q8YWM0	strain PCC7120 (ATCC 27893)	-

Oxidation Stability

Oxidation Stability	Organism
the enzyme retains more than 50% of its activity at 2.5 mM H2O2	Anabaena sp.

Purification (Commentary)

Purification (Comment)	Organism
Toyopearl DEAE-650M column chromatography, Toyopearl butyl-650M column chromatography, and hydroxylapatite column chromatography	Anabaena sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
5.6	-	recombinant enzyme from crude extract, using Reactive Blue 5 as substrate, pH 4.0-4.4, at 37°C	Anabaena sp.
54	-	recombinant enzyme, in 50 mM citrate buffer (pH 4.0-4.4), at 37°C, using syringaldehyde as substrate	Anabaena sp.
71	-	recombinant enzyme, in 50 mM citrate buffer (pH 4.0-4.4), at 37°C, using 2,6-dimethoxyphenol as substrate	Anabaena sp.
204	-	recombinant enzyme, in 50 mM citrate buffer (pH 4.0-4.4), at 37°C, using 2,2'-azino-bis(3-ethylbenzthiazoline)-6-sulfonic acid as substrate	Anabaena sp.
216	-	recombinant enzyme, in 50 mM citrate buffer (pH 4.0-4.4), at 37°C, using pyrogallol as substrate	Anabaena sp.
230	-	recombinant enzyme, in 50 mM citrate buffer (pH 4.0-4.4), at 37°C, using guaiacol as substrate	Anabaena sp.
283	-	recombinant enzyme after 50.5fold purification, using Reactive Blue 5 as substrate, pH 4.0-4.4, at 37°C	Anabaena sp.
290	-	recombinant enzyme, in 50 mM citrate buffer (pH 4.0-4.4), at 37°C, using L-ascorbate as substrate	Anabaena sp.
308	-	recombinant enzyme, in 50 mM citrate buffer (pH 4.0-4.4), at 37°C, using D-isoascorbate as substrate	Anabaena sp.
308	-	recombinant enzyme, in 50 mM citrate buffer (pH 4.0-4.4), at 37°C, using NADPH as substrate	Anabaena sp.
374	-	recombinant enzyme, in 50 mM citrate buffer (pH 4.0-4.4), at 37°C, using NADH as substrate	Anabaena sp.
1478	-	recombinant enzyme, in 50 mM citrate buffer (pH 4.0-4.4), at 37°C, using 4-aminoantipyrine as substrate	Anabaena sp.

Storage Stability

Storage Stability	Organism
4°C, 50 mM potassium phosphate buffer (pH 7.0), 40 days, 10% loss of activity	Anabaena sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
2,2'-azino-bis(3-ethylbenzthiazoline)-6-sulfonic acid + H2O2 + H+	89% activity compared to guaiacol	Anabaena sp.	?	-	?
2,6-dimethoxyphenol + H2O2 + H+	31% activity compared to guaiacol	Anabaena sp.	?	-	?
4-aminoantipyrine + H2O2 + H+	643% activity compared to guaiacol	Anabaena sp.	?	-	?
Acid Blue 45 + H2O2 + H+	98% decolorization rate compared to Reactive Blue 5	Anabaena sp.	oxidized Acid Blue 45 + H2O	-	?
Acid Red 151 + H2O2 + H+	12% decolorization rate compared to Reactive Blue 5	Anabaena sp.	oxidized Acid Red 151 + H2O	-	?
Congo Red + H2O2 + H+	12% decolorization rate compared to Reactive Blue 5	Anabaena sp.	oxidized Congo Red + H2O	-	?
D-isoascorbate + H2O2 + H+	134% activity compared to guaiacol	Anabaena sp.	?	-	?
Direct Sky Blue 6B + H2O2 + H+	50% decolorization rate compared to Reactive Blue 5	Anabaena sp.	oxidized Direct Sky Blue 6B + H2O	-	?
guaiacol + H2O2 + H+	100% activity	Anabaena sp.	?	-	?
L-ascorbate + H2O2 + H+	126% activity compared to guaiacol	Anabaena sp.	?	-	?
additional information	the decolorization activity of AnaPX toward azo dyes is relatively low. No decolorization of Toluidine Blue O, Reactive Red 33, and Reactive Yellow 2 is observed	Anabaena sp.	?	-	?
Procion Blue H-ERD + H2O2 + H+	90% decolorization rate compared to Reactive Blue 5	Anabaena sp.	oxidized Procion Blue H-ERD + H2O	-	?
Procion Blue H-EXL + H2O2 + H+	72% decolorization rate compared to Reactive Blue 5	Anabaena sp.	oxidized Procion Blue H-EXL + H2O	-	?
pyrogallol + H2O2 + H+	93% activity compared to guaiacol	Anabaena sp.	?	-	?
Reactive Black 5 + H2O2 + H+	8% decolorization rate compared to Reactive Blue 5	Anabaena sp.	oxidized Reactive Black 5 + H2O	-	?
Reactive Blue 114 + H2O2 + H+	187% decolorization rate compared to Reactive Blue 5	Anabaena sp.	oxidized Reactive Blue 114 + H2O	-	?
Reactive Blue 19 + H2O2 + H+	153% decolorization rate compared to Reactive Blue 5	Anabaena sp.	oxidized Reactive Blue 19 + H2O	-	?
Reactive Blue 4 + H2O2 + H+	64% decolorization rate compared to Reactive Blue 5	Anabaena sp.	oxidized Reactive Blue 4 + H2O	-	?
Reactive Blue 5 + H2O2 + H+	123% activity compared to guaiacol, the apparent optimum concentration of H2O2 required for the decolorization of Reactive Blue 5 by AnaPX is 0.4 mM	Anabaena sp.	oxidized Reactive Blue 5 + H2O	-	?
Reactive Green 19 + H2O2 + H+	35% decolorization rate compared to Reactive Blue 5	Anabaena sp.	oxidized Reactive Green 19 + H2O	-	?
Reactive Orange 14 + H2O2 + H+	10% decolorization rate compared to Reactive Blue 5	Anabaena sp.	oxidized Reactive Orange 14 + H2O	-	?
Reactive Red 120 + H2O2 + H+	5% decolorization rate compared to Reactive Blue 5	Anabaena sp.	oxidized Reactive Red 120 + H2O	-	?
Reactive Yellow 86 + H2O2 + H+	3% decolorization rate compared to Reactive Blue 5	Anabaena sp.	oxidized Reactive Yellow 86 + H2O	-	?
syringaldehyde + H2O2 + H+	23% activity compared to guaiacol	Anabaena sp.	oxidized syringaldehyde + H2O	-	?

Subunits

Subunits	Comment	Organism
homotetramer	4 * 53280, native enzyme, MALDI-TOF spectrometry	Anabaena sp.
homotetramer	4 * 53368, deduced from amino acid sequence	Anabaena sp.
homotetramer	4 * 54000, native enzyme, SDS-PAGE	Anabaena sp.

Synonyms

Synonyms	Comment	Organism
AnaPX	-	Anabaena sp.

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
35	-	-	Anabaena sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
30	60	the enzyme is considerably stable at 30°C and 40°C where it retains more than 90% of its activity, while the enzyme loses more than 90% of its activity after incubation at 50°C and 60°C for 3 h	Anabaena sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
384	-	H2O2	in 50 mM citrate buffer (pH 4.0-4.4), at 37°C	Anabaena sp.
384	-	Reactive Blue 5	in 50 mM citrate buffer (pH 4.0-4.4), at 37°C	Anabaena sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4	4.4	-	Anabaena sp.

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
3.5	9.5	when maintained at 40°C for 20 min, the enzyme is stable at pH values between 3.5 and 9.5. The enzyme shows 20% relative activity at pH 3.0 and 10.0, 40% relative activity at pH 4.0, about 65% relative activity at pH 4.5 and 9.0, about 80% relative activity at pH 5.0, more than 90% relative activity at pH 6.0-8.0	Anabaena sp.

Cofactor

Cofactor	Comment	Organism	Structure
heme	iron protoporphyrin-containing heme peroxidase, the heme content is estimated to be 0.91 mol per mole of protein	Anabaena sp.

pI Value

Organism	Comment	pI Value Maximum	pI Value
Anabaena sp.	isoelectric focusing	-	3.7

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
66000	-	H2O2	in 50 mM citrate buffer (pH 4.0-4.4), at 37°C	Anabaena sp.
120000	-	Reactive Blue 5	in 50 mM citrate buffer (pH 4.0-4.4), at 37°C	Anabaena sp.