Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
vanadate | in the solid state structure, every protein monomer binds one vanadate to the tele imidazole nitrogen of residue His486. In solutions of sodium orthovanadate, isoform apobromoperoxidase II recovers bromoperoxidase activity by one order of magnitude faster than apobromoperoxidase I | Ascophyllum nodosum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67400 | - |
2 * 67400, calculated, 2 * 64200-65000, MALDI-TOF | Ascophyllum nodosum |
127700 | 130000 | MALDI-TOF | Ascophyllum nodosum |
136000 | - |
gel filtration | Ascophyllum nodosum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ascophyllum nodosum | P81701 | isoform VBrPO(AnI) | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Br- + H2O2 + 2-methoxyphenol | - |
Ascophyllum nodosum | 2-bromo-6-methoxyphenol + 4-bromo-6-methoxyphenol + H2O | 56% of product, in a 21/79 mixture of o-/p-regioisomers, plus 10% 2,4-dibromo-6-methoxyphenol | ? | |
Br- + H2O2 + 2-methylphenol | - |
Ascophyllum nodosum | 2-bromo-6-methylphenol + 4-bromo-6-methylphenol + H2O | 68% of product, in a 16/84 mixture of o-/p-regioisomers, plus 4% 2,4-dibromophenol | ? | |
Br- + H2O2 + 2-t-butylphenol | - |
Ascophyllum nodosum | 2-bromo-6-t-butylphenol + 4-bromo-6-t-butylphenol + H2O | 42% of product, in a 36/64 mixture of o-/p-regioisomers, plus 2% 2,4-dibromo-6-t-butylphenol | ? | |
Br- + H2O2 + phenol | - |
Ascophyllum nodosum | 2-bromophenol + 4-bromophenol + H2O | 69% of product, in a 91/9 mixture of o-/p-regioisomers, plus 3% 2,4-dibromo-6-methylphenol and some 2,4,6-tribromophenol | ? | |
additional information | enzyme uses hydrogen peroxide and bromide yielding molecular bromine as reagent for electrophilic hydrocarbon bromination | Ascophyllum nodosum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 67400, calculated, 2 * 64200-65000, MALDI-TOF | Ascophyllum nodosum |
Synonyms | Comment | Organism |
---|---|---|
apobromoperoxidase I | - |
Ascophyllum nodosum |
VBrPO(AnI) | - |
Ascophyllum nodosum |