Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Ecklonia cava subsp. stolonifera | |
Fe2+ | - |
Ecklonia cava subsp. stolonifera | |
Fe3+ | - |
Ecklonia cava subsp. stolonifera |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00012 | - |
monochlorodimedone | pH 6, 25°C | Ecklonia cava subsp. stolonifera | |
0.001 | - |
Br- | pH 6, 25°C | Ecklonia cava subsp. stolonifera | |
0.052 | - |
H2O2 | pH 6, 25°C | Ecklonia cava subsp. stolonifera |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Vanadium | requires vanadium for enzyme activity. The enzyme activity increases ca. 250% with the action of V5+ on the isolated enzyme, since more than 2/3 of the protein molecules are in the apo form. This effect of V5+ addition is inhibited in phosphate buffer, probably because phosphate and vanadate compete for the active site | Ecklonia cava subsp. stolonifera |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83000 | - |
x * 83000, SDS-PAGE | Ecklonia cava subsp. stolonifera |
Organic Solvent | Comment | Organism |
---|---|---|
1-propanol | completely intact in the presence of 50% methanol | Ecklonia cava subsp. stolonifera |
Ethanol | completely intact in the presence of 50% methanol | Ecklonia cava subsp. stolonifera |
Methanol | completely intact in the presence of 50% methanol | Ecklonia cava subsp. stolonifera |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ecklonia cava subsp. stolonifera | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Ecklonia cava subsp. stolonifera |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
32.5 | - |
- |
Ecklonia cava subsp. stolonifera |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione | i.e. monochlorodimedone | Ecklonia cava subsp. stolonifera | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 83000, SDS-PAGE | Ecklonia cava subsp. stolonifera |
Synonyms | Comment | Organism |
---|---|---|
vanadium bromoperoxidase | - |
Ecklonia cava subsp. stolonifera |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
68 | - |
Tm-value | Ecklonia cava subsp. stolonifera |
80 | - |
10 min, complete loss of activity | Ecklonia cava subsp. stolonifera |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Ecklonia cava subsp. stolonifera |