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Literature summary for 1.11.1.16 extracted from

  • Marques, G.; Gamelas, J.A.; Ruiz-Duenas, F.J.; del Rio, J.C.; Evtuguin, D.V.; Martinez, A.T.; Gutierrez, A.
    Delignification of eucalypt kraft pulp with manganese-substituted polyoxometalate assisted by fungal versatile peroxidase (2010), Biores. Technol., 101, 5935-5940.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
H2O2
-
Pleurotus eryngii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4
-
manganese(II)-substituted polyoxometalate in 0.1 M sodium tartrate, pH 5.0, at 20°C Pleurotus eryngii

Organism

Organism UniProt Comment Textmining
Pleurotus eryngii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Resource-Q column chromatography Pleurotus eryngii

Renatured (Commentary)

Renatured (Comment) Organism
using 0.15 M urea, 5 mM Ca2+, 0.02 mM hemin, a 4:1 oxidized-glutathione/reduced-glutathione ratio and 0.1 mg/ml protein at pH 9.5 Pleurotus eryngii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
manganese(II)-substituted polyoxometalate + H2O2
-
Pleurotus eryngii manganese(III)-substituted polyoxometalate + H2O2
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
47
-
manganese(II)-substituted polyoxometalate in 0.1 M sodium tartrate, pH 5.0, at 20°C Pleurotus eryngii

Cofactor

Cofactor Comment Organism Structure
heme
-
Pleurotus eryngii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
7.36
-
manganese(II)-substituted polyoxometalate in 0.1 M sodium tartrate, pH 5.0, at 20°C Pleurotus eryngii