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Literature summary for 1.11.1.14 extracted from

  • Ryu, K.; Kang, J.H.; Wang, L.; Lee, E.K.
    Expression in yeast of secreted lignin peroxidase with improved 2,4-dichlorophenol degradability by DNA shuffling (2008), J. Biotechnol., 135, 241-246.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Phanerodontia chrysosporium

Protein Variants

Protein Variants Comment Organism
A140G/S190P/P193A/S196F/E208Q the variant shows increased 2,4-dichlorophenol degradation activity (ca. 1.6fold) and stability against H2O2. Kcat for H2O2 increases over the wild type value by about 6.5fold, the Km values for H2O2 is lower than wild type value Phanerodontia chrysosporium
P106R/S119R/N120T/S228Y/A272G/L275V/A315G/A317T the variant shows increased 2,4-dichlorophenol degradation activity (ca. 1.6fold) and stability against H2O2. Kcat for H2O2 increases over the wild type value by about 6.5fold, the Km values for H2O2 is lower than wild type value Phanerodontia chrysosporium
S274L/L275F/A292 the variant shows increased 2,4-dichlorophenol degradation activity (ca. 1.6fold) and stability against H2O2. Kcat for H2O2 increases over the wild type value by about 6.5fold, the Km values for H2O2 is lower than wild type value Phanerodontia chrysosporium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.163
-
2,4-Dichlorophenol mutant enzyme A140G/S190P/P193A/S196F/E208Q Phanerodontia chrysosporium
0.179
-
2,4-Dichlorophenol mutant enzyme S274L/L275F/A292 Phanerodontia chrysosporium
0.19
-
2,4-Dichlorophenol mutant enzyme P106R/S119R/N120T/S228Y/A272G/L275V/A315G/A317T Phanerodontia chrysosporium
0.359
-
2,4-Dichlorophenol wild-type enzyme Phanerodontia chrysosporium
4.09
-
H2O2 mutant enzyme P106R/S119R/N120T/S228Y/A272G/L275V/A315G/A317T Phanerodontia chrysosporium
4.32
-
H2O2 mutant enzyme S274L/L275F/A292 Phanerodontia chrysosporium
4.47
-
H2O2 mutant enzyme A140G/S190P/P193A/S196F/E208Q Phanerodontia chrysosporium
23.6
-
H2O2 wild-type enzyme Phanerodontia chrysosporium

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dichlorophenol + H2O2
-
Phanerodontia chrysosporium ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10.83
-
H2O2 wild-type enzyme Phanerodontia chrysosporium
37.1
-
2,4-Dichlorophenol wild-type enzyme Phanerodontia chrysosporium
58.7
-
2,4-Dichlorophenol mutant enzyme A140G/S190P/P193A/S196F/E208Q Phanerodontia chrysosporium
59.7
-
2,4-Dichlorophenol mutant enzyme S274L/L275F/A292 Phanerodontia chrysosporium
66
-
2,4-Dichlorophenol mutant enzyme P106R/S119R/N120T/S228Y/A272G/L275V/A315G/A317T Phanerodontia chrysosporium
68.2
-
H2O2 mutant enzyme P106R/S119R/N120T/S228Y/A272G/L275V/A315G/A317T Phanerodontia chrysosporium
72
-
H2O2 mutant enzyme S274L/L275F/A292 Phanerodontia chrysosporium
74.5
-
H2O2 mutant enzyme A140G/S190P/P193A/S196F/E208Q Phanerodontia chrysosporium