Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae | Phanerodontia chrysosporium |
Protein Variants | Comment | Organism |
---|---|---|
A140G/S190P/P193A/S196F/E208Q | the variant shows increased 2,4-dichlorophenol degradation activity (ca. 1.6fold) and stability against H2O2. Kcat for H2O2 increases over the wild type value by about 6.5fold, the Km values for H2O2 is lower than wild type value | Phanerodontia chrysosporium |
P106R/S119R/N120T/S228Y/A272G/L275V/A315G/A317T | the variant shows increased 2,4-dichlorophenol degradation activity (ca. 1.6fold) and stability against H2O2. Kcat for H2O2 increases over the wild type value by about 6.5fold, the Km values for H2O2 is lower than wild type value | Phanerodontia chrysosporium |
S274L/L275F/A292 | the variant shows increased 2,4-dichlorophenol degradation activity (ca. 1.6fold) and stability against H2O2. Kcat for H2O2 increases over the wild type value by about 6.5fold, the Km values for H2O2 is lower than wild type value | Phanerodontia chrysosporium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.163 | - |
2,4-Dichlorophenol | mutant enzyme A140G/S190P/P193A/S196F/E208Q | Phanerodontia chrysosporium | |
0.179 | - |
2,4-Dichlorophenol | mutant enzyme S274L/L275F/A292 | Phanerodontia chrysosporium | |
0.19 | - |
2,4-Dichlorophenol | mutant enzyme P106R/S119R/N120T/S228Y/A272G/L275V/A315G/A317T | Phanerodontia chrysosporium | |
0.359 | - |
2,4-Dichlorophenol | wild-type enzyme | Phanerodontia chrysosporium | |
4.09 | - |
H2O2 | mutant enzyme P106R/S119R/N120T/S228Y/A272G/L275V/A315G/A317T | Phanerodontia chrysosporium | |
4.32 | - |
H2O2 | mutant enzyme S274L/L275F/A292 | Phanerodontia chrysosporium | |
4.47 | - |
H2O2 | mutant enzyme A140G/S190P/P193A/S196F/E208Q | Phanerodontia chrysosporium | |
23.6 | - |
H2O2 | wild-type enzyme | Phanerodontia chrysosporium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phanerodontia chrysosporium | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dichlorophenol + H2O2 | - |
Phanerodontia chrysosporium | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.83 | - |
H2O2 | wild-type enzyme | Phanerodontia chrysosporium | |
37.1 | - |
2,4-Dichlorophenol | wild-type enzyme | Phanerodontia chrysosporium | |
58.7 | - |
2,4-Dichlorophenol | mutant enzyme A140G/S190P/P193A/S196F/E208Q | Phanerodontia chrysosporium | |
59.7 | - |
2,4-Dichlorophenol | mutant enzyme S274L/L275F/A292 | Phanerodontia chrysosporium | |
66 | - |
2,4-Dichlorophenol | mutant enzyme P106R/S119R/N120T/S228Y/A272G/L275V/A315G/A317T | Phanerodontia chrysosporium | |
68.2 | - |
H2O2 | mutant enzyme P106R/S119R/N120T/S228Y/A272G/L275V/A315G/A317T | Phanerodontia chrysosporium | |
72 | - |
H2O2 | mutant enzyme S274L/L275F/A292 | Phanerodontia chrysosporium | |
74.5 | - |
H2O2 | mutant enzyme A140G/S190P/P193A/S196F/E208Q | Phanerodontia chrysosporium |