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Literature summary for 1.11.1.13 extracted from

  • Silva, E.; Martins, S.; Milagres, A.
    Extraction of manganese peroxidase produced by Lentinula edodes (2008), Biores. Technol., 99, 2471-2475.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Manganese
-
Lentinula edodes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Mn2+ + H+ + H2O2 Lentinula edodes
-
Mn3+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Lentinula edodes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
enzymes are extracted with 50 mM citrate-phosphate buffer at pH 4.0, 5.0 and 6.0 at 10°C and 25°C Lentinula edodes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.01
-
specific MnP activity recovered from solid-state culture after 1h and 10°C, U/mg of fugal biomass, pH 6.0 Lentinula edodes
0.01
-
specific MnP activity recovered from solid-state culture after 1h and 25°C, U/mg of fugal biomass, pH 6.0 Lentinula edodes
0.04
-
effect of dialysis on MnP recovered from solid-state culture at 10°C, dialyzed extract, extraction conducted for 3h at 100 rpm, U/mg of fugal biomass, pH 4.0 Lentinula edodes
0.1
-
specific MnP activity recovered from solid-state culture after 3h and 10°C, U/mg of fugal biomass, pH 6.0 Lentinula edodes
0.12
-
specific MnP activity recovered from solid-state culture after 3h and 25°C, U/mg of fugal biomass, pH 6.0 Lentinula edodes
0.16
-
effect of dialysis on MnP recovered from solid-state culture at 10°C, dialyzed extract, extraction conducted for 1h without agitation, U/mg of fugal biomass, pH 5.0 Lentinula edodes
0.2
-
specific MnP activity recovered from solid-state culture after 1h and 25°C, U/mg of fugal biomass, pH 4.0 Lentinula edodes
0.21
-
effect of dialysis on MnP recovered from solid-state culture at 10°C, dialyzed extract, extraction conducted for 3h at 100 rpm, U/mg of fugal biomass, pH 5.0 Lentinula edodes
0.27
-
effect of dialysis on MnP recovered from solid-state culture at 10°C, dialyzed extract, extraction conducted for 1h without agitation, U/mg of fugal biomass, pH 4.0 Lentinula edodes
0.31
-
effect of dialysis on MnP recovered from solid-state culture at 10°C, crude extract, extraction conducted for 3h at 100 rpm, U/mg of fugal biomass, pH 4.0 Lentinula edodes
0.31
-
specific MnP activity recovered from solid-state culture after 3h and 10°C, U/mg of fugal biomass, pH 4.0 Lentinula edodes
0.35
-
effect of dialysis on MnP recovered from solid-state culture at 10°C, crude extract, extraction conducted for 1h without agitation, U/mg of fugal biomass, pH 4.0 Lentinula edodes
0.35
-
effect of dialysis on MnP recovered from solid-state culture at 10°C, crude extract, extraction conducted for 1h without agitation, U/mg of fugal biomass, pH 5.0 Lentinula edodes
0.35
-
specific MnP activity recovered from solid-state culture after 1h and 10°C, U/mg of fugal biomass, pH 4.0 Lentinula edodes
0.35
-
specific MnP activity recovered from solid-state culture after 1h and 10°C, U/mg of fugal biomass, pH 5.0 Lentinula edodes
0.41
-
effect of dialysis on MnP recovered from solid-state culture at 10°C, crude extract, extraction conducted for 3h at 100 rpm, U/mg of fugal biomass, pH 5.0 Lentinula edodes
0.41
-
specific MnP activity recovered from solid-state culture after 3h and 10°C, U/mg of fugal biomass, pH 5.0 Lentinula edodes
0.45
-
specific MnP activity recovered from solid-state culture after 1h and 25°C, U/mg of fugal biomass, pH 5.0 Lentinula edodes
0.48
-
specific MnP activity recovered from solid-state culture after 3h and 25°C, U/mg of fugal biomass, pH 4.0 Lentinula edodes
0.7
-
specific MnP activity recovered from solid-state culture after 3h and 25°C, U/mg of fugal biomass, pH 5.0 Lentinula edodes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Mn2+ + H+ + H2O2
-
Lentinula edodes Mn3+ + H2O
-
?
phenol red activity assay Lentinula edodes ?
-
?

Synonyms

Synonyms Comment Organism
manganese peroxidase
-
Lentinula edodes
MnP
-
Lentinula edodes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
Lentinula edodes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Lentinula edodes

pH Range

pH Minimum pH Maximum Comment Organism
4 6
-
Lentinula edodes