General Stability | Organism |
---|---|
enzyme is very stable when the molar concentration of H2O2 is 10times of the concentration of the enzyme. When the H2O2 concentration is 250fold and 1000fold of the MnP concentration, the MnP activity decreases by 14% and 45%, respectively, in the first 15 min and remains the same thereafter. When the H2O2 concentration is 5000times of the enzyme concentration, the enzyme activity rapidly decreases in 30 min and then continues to decrease, but at a slower rate | Trichophyton rubrum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0064 | - |
H2O2 | reaction with Mn2+ | Trichophyton rubrum | |
0.063 | - |
Mn2+ | reaction with H2O2 | Trichophyton rubrum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 2,2'-azino-bis(3-ethyl-benzothiazoline)-6-sulfonic acid, guaiacol and 2,6-dimethoxyphenol are oxidized at a faster rate in presence of Mn(II) than in absence of Mn(II) | Trichophyton rubrum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
x * 42000, SDS-PAGE | Trichophyton rubrum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichophyton rubrum | - |
LSK-27 | - |
Trichophyton rubrum LSK-27 | - |
LSK-27 | - |
Purification (Comment) | Organism |
---|---|
- |
Trichophyton rubrum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 + 2,2'-azino-bis(3-ethyl)-benzothiazoline-6-sulfonic acid | oxidized at a faster rate in presence of Mn(II) than in absence of Mn(II) | Trichophyton rubrum | H2O + ? | - |
? | |
H2O2 + 2,2'-azino-bis(3-ethyl)-benzothiazoline-6-sulfonic acid | oxidized at a faster rate in presence of Mn(II) than in absence of Mn(II) | Trichophyton rubrum LSK-27 | H2O + ? | - |
? | |
H2O2 + 2,6-dimethoxyphenol | oxidized at a faster rate in presence of Mn(II) than in absence of Mn(II) | Trichophyton rubrum | H2O + ? | - |
? | |
H2O2 + 2,6-dimethoxyphenol | oxidized at a faster rate in presence of Mn(II) than in absence of Mn(II) | Trichophyton rubrum LSK-27 | H2O + ? | - |
? | |
H2O2 + guaiacol | oxidized at a faster rate in presence of Mn(II) than in absence of Mn(II) | Trichophyton rubrum | H2O + ? | - |
? | |
H2O2 + guaiacol | oxidized at a faster rate in presence of Mn(II) than in absence of Mn(II) | Trichophyton rubrum LSK-27 | H2O + ? | - |
? | |
Mn2+ + H2O2 | - |
Trichophyton rubrum | Mn3+ + H2O | - |
? | |
Mn2+ + H2O2 | - |
Trichophyton rubrum LSK-27 | Mn3+ + H2O | - |
? | |
additional information | no oxidation of veratryl alcohol | Trichophyton rubrum | ? | - |
? | |
additional information | no oxidation of veratryl alcohol | Trichophyton rubrum LSK-27 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, SDS-PAGE | Trichophyton rubrum |
Synonyms | Comment | Organism |
---|---|---|
MnP | - |
Trichophyton rubrum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
60 min, stable | Trichophyton rubrum |
50 | - |
20% decrease of activity after 20 min, no further loss of activity after 60 min | Trichophyton rubrum |
60 | - |
80% loss of activity after 10 min, complete inactivation after 40 min | Trichophyton rubrum |
70 | - |
complete inactivation after 5 min | Trichophyton rubrum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
272.4 | - |
H2O2 | reaction with Mn2+ | Trichophyton rubrum | |
304.8 | - |
Mn2+ | reactioin with H2O2 | Trichophyton rubrum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Trichophyton rubrum | isoelectric focusing | - |
8.2 |