Application | Comment | Organism |
---|---|---|
environmental protection | mediated system of degradation is potentially valuable for degradation of synthetic polymers and of environmental pollutants | Phanerodontia chrysosporium |
paper production | mediated system of degradation is potentially valuable for pulp and paper industries | Phanerodontia chrysosporium |
Cloned (Comment) | Organism |
---|---|
expression of A48C/A63C double mutant mnp gene in Escherichia coli XL-1 Blue | Phanerodontia chrysosporium |
Crystallization (Comment) | Organism |
---|---|
crystal structure | Phanerodontia chrysosporium |
Protein Variants | Comment | Organism |
---|---|---|
A48C/A63C | A48C and A63C double mutant with an engineered disulfide bond near the distal calcium binding site to restrict the movement of helix B upon loss of calcium and to stabilize against this loss, thermal and pH-stability is improved compared with that of native and recombinant MnP, thermally treated enzyme contains one calcium and retains a percentage of its activity | Phanerodontia chrysosporium |
General Stability | Organism |
---|---|
calcium stabilizes | Phanerodontia chrysosporium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | MnP calcium binding site, calcium content: 4 mol per mol of native MnP, 2 mol per mol of recombinant and A48C/A63C double mutant MnP, calcium decreases the rate of thermal inactivation | Phanerodontia chrysosporium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phanerodontia chrysosporium | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | glycosylation stabilizes MnP | Phanerodontia chrysosporium |
Purification (Comment) | Organism |
---|---|
isoenzyme H4 | Phanerodontia chrysosporium |
purification of recombinant A48C/A63C double mutant MnP, expressed in Escherichia coli | Phanerodontia chrysosporium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Mn2+ + H+ + H2O2 | - |
Phanerodontia chrysosporium | Mn3+ + H2O | - |
? | |
additional information | structural properties | Phanerodontia chrysosporium | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
susceptible to thermal inactivation due to the loss of calcium, calcium content after thermal treatment at 37°C for 15 min: 1.5 mol per mol of native MnP, 1 mol per mol of recombinant MnP and 1.3 mol per mol of A48C/A63C double mutant MnP, biphasic inactivation kinetics, recombinant MnP is more sensitive than native MnP as a result of the lack of glycosylation, calcium decreases the rate of thermal inactivation and reactivates MnP up to 50% of its original activity, while EGTA increases the rate of inactivation | Phanerodontia chrysosporium |
37 | - |
recombinant MnP: 5 min, 50% loss of activity, A48C/A63C double mutant MnP: relatively stable | Phanerodontia chrysosporium |
52 | - |
recombinant MnP: 20 s, 50% loss of activity, A48C/A63C double mutant MnP: 2 min, 50% loss of activity | Phanerodontia chrysosporium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
24 | - |
Mn2+ | A48C/A63C double mutant MnP after thermal treatment at 37°C for 15 min | Phanerodontia chrysosporium | |
218 | - |
Mn2+ | native MnP | Phanerodontia chrysosporium | |
270 | 273 | Mn2+ | recombinant MnP and A48C/A63C double mutant MnP | Phanerodontia chrysosporium |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
recombinant MnP is more sensitive than native MnP as a result of lack of glycosylation | Phanerodontia chrysosporium |
4.5 | - |
recombinant MnP, native MnP and A48C/A63C double mutant MnP are stable | Phanerodontia chrysosporium |
6 | - |
native and A48C/A63C double mutant MnP: 1 h, less than 40% loss of activity | Phanerodontia chrysosporium |
7 | - |
native MnP: 1 h, 80% loss of activity, A48C/A63C double mutant MnP: 1 h, 60% loss of activity | Phanerodontia chrysosporium |
8 | - |
recombinant MnP: inactivation within 1 min, native MnP: inactivation within 15 min, A48C/A63C double mutant MnP: 1 h, 80% loss of activity | Phanerodontia chrysosporium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | enzyme contains a pentacoordinated, essentially high-spin ferric heme | Phanerodontia chrysosporium | |
heme | heme protein | Phanerodontia chrysosporium |