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Literature summary for 1.11.1.13 extracted from
- Purification, characterization, and chemical modification of manganese peroxidase from Bjerkandera adusta UAMH 8258 (2002), Curr. Microbiol., 45, 77-87.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| H2O2 | highest Mn2+-oxidation activity when the H2O2 concentration is 0.1 mM | Bjerkandera adusta |  |
Application
| Application | Comment | Organism |
|---|---|---|
| environmental protection | polycyclic aromatic hydrocarbon degradation | Bjerkandera adusta |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00366 | - |
2,6-dimethoxyphenol | Mn2+-dependent activity | Bjerkandera adusta | |
| 0.0045 | - |
H2O2 | - |
Bjerkandera adusta | |
| 0.006 | 0.009 | H2O2 | - |
Pleurotus eryngii | |
| 0.0125 | - |
2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate | Mn2+-independent activity | Bjerkandera adusta | |
| 0.015 | - |
2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate | Mn2+-dependent activity | Bjerkandera adusta | |
| 0.017 | - |
Mn2+ | - |
Bjerkandera adusta | |
| 0.02 | - |
Mn2+ | below | Pleurotus eryngii | |
| 0.1445 | - |
2,6-dimethoxyphenol | Mn2+-independent activity | Bjerkandera adusta | |
| 0.222 | - |
guaiacol | Mn2+-dependent activity | Bjerkandera adusta | |
| 0.3 | - |
2,6-dimethoxyphenol | - |
Pleurotus eryngii | |
| 0.432 | - |
veratryl alcohol | Mn2+-dependent activity | Bjerkandera adusta | |
| 3 | - |
veratryl alcohol | - |
Pleurotus eryngii | |
| 4.96 | - |
guaiacol | Mn2+-independent activity | Bjerkandera adusta | |
| 5.3 | - |
veratryl alcohol | Mn2+-independent activity | Bjerkandera adusta | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bjerkandera adusta | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36600 | - |
x * 36600, MALDI-TOF mass spectrometric analysis, more accurate reflection of the true molecular weight than by SDS-PAGE | Bjerkandera adusta |
| 43000 | - |
x * 43000, SDS-PAGE | Bjerkandera adusta |
| 43000 | - |
x * 43000, SDS-PAGE | Pleurotus eryngii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bjerkandera adusta | - |
- |
- |
| Bjerkandera adusta UAMH 8258 | - |
- |
- |
| Pleurotus eryngii | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Bjerkandera adusta |
| glycoprotein | - |
Pleurotus eryngii |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 24fold purification | Bjerkandera adusta |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bjerkandera adusta |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate + H+ + H2O2 | - |
Bjerkandera adusta | ? | - |
r | |
| 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate + Mn2+ + H2O2 | - |
Bjerkandera adusta | ? | - |
r | |
| 2,6-dimethoxyphenol + H+ + H2O2 | - |
Bjerkandera adusta | ? | - |
r | |
| 2,6-dimethoxyphenol + H+ + H2O2 | - |
Pleurotus eryngii | ? | - |
r | |
| guaiacol + H+ + H2O2 | - |
Bjerkandera adusta | ? | - |
r | |
| guaiacol + Mn2+ + H2O2 | - |
Bjerkandera adusta | ? | - |
r | |
| Mn2+ + H+ + H2O2 | - |
Bjerkandera adusta | Mn3+ + H2O | - |
? | |
| Mn2+ + H+ + H2O2 | - |
Pleurotus eryngii | Mn3+ + H2O | - |
? | |
| Mn2+ + H+ + H2O2 | - |
Bjerkandera adusta UAMH 8258 | Mn3+ + H2O | - |
? | |
| additional information | - |
Pleurotus eryngii | ? | - |
? | |
| additional information | Mn2+-dependent and Mn2+-independent peroxidase activities, substrates: 2,6-dimethoxyphenol, 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate, guaiacol and veratryl alcohol | Bjerkandera adusta | ? | - |
? | |
| additional information | enzyme oxidizes 4-aminophenol and hydroquinone | Bjerkandera adusta | ? | - |
? | |
| additional information | enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate | Bjerkandera adusta | ? | - |
? | |
| additional information | enzyme oxidizes 2,6-dimethoxyphenol | Bjerkandera adusta | ? | - |
? | |
| additional information | Mn2+-dependent and Mn2+-independent peroxidase activities, substrates: 2,6-dimethoxyphenol, 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate, guaiacol and veratryl alcohol | Bjerkandera adusta UAMH 8258 | ? | - |
? | |
| additional information | enzyme oxidizes 4-aminophenol and hydroquinone | Bjerkandera adusta UAMH 8258 | ? | - |
? | |
| additional information | enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate | Bjerkandera adusta UAMH 8258 | ? | - |
? | |
| additional information | enzyme oxidizes 2,6-dimethoxyphenol | Bjerkandera adusta UAMH 8258 | ? | - |
? | |
| veratryl alcohol + H+ + H2O2 | - |
Bjerkandera adusta | ? | - |
r | |
| veratryl alcohol + H+ + H2O2 | - |
Pleurotus eryngii | ? | - |
r | |
| veratryl alcohol + Mn2+ + H2O2 | - |
Bjerkandera adusta | ? | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000, SDS-PAGE | Bjerkandera adusta |
| ? | x * 43000, SDS-PAGE | Pleurotus eryngii |
| ? | x * 36600, MALDI-TOF mass spectrometric analysis, more accurate reflection of the true molecular weight than by SDS-PAGE | Bjerkandera adusta |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
highest Mn2+ oxidation rate over 1 min | Bjerkandera adusta |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
polyethylene glycol-modified enzyme shows greater stability to higher temperatures than native enzyme | Bjerkandera adusta |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.33 | - |
veratryl alcohol | Mn2+-independent activity | Bjerkandera adusta | |
| 14.5 | - |
guaiacol | Mn2+-independent activity | Bjerkandera adusta | |
| 31.7 | - |
guaiacol | Mn2+-dependent activity | Bjerkandera adusta | |
| 39.5 | - |
veratryl alcohol | Mn2+-dependent activity | Bjerkandera adusta | |
| 70 | - |
2,6-dimethoxyphenol | Mn2+-independent activity | Bjerkandera adusta | |
| 120 | - |
2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate | Mn2+-dependent activity | Bjerkandera adusta | |
| 139 | - |
2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate | Mn2+-independent activity | Bjerkandera adusta | |
| 150 | - |
Mn2+ | - |
Bjerkandera adusta | |
| 225 | - |
H2O2 | - |
Bjerkandera adusta | |
| 320 | - |
2,6-dimethoxyphenol | Mn2+-dependent activity | Bjerkandera adusta | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pleurotus eryngii |
| additional information | - |
pI: 3.5 | Bjerkandera adusta |
| 3 | - |
- |
Pleurotus eryngii |
| 3 | - |
Mn2+-independent peroxidase activity | Bjerkandera adusta |
| 5 | - |
- |
Pleurotus eryngii |
| 5 | - |
Mn2+-dependent peroxidase activity | Bjerkandera adusta |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
polyethylene glycol-modified enzyme shows greater stability to lower pH than native enzyme | Bjerkandera adusta |
| 10 | - |
enzyme loses activity rapidly at pH 10 | Bjerkandera adusta |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | heme protein | Bjerkandera adusta | |
| heme | heme protein | Pleurotus eryngii | |
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