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Literature summary for 1.11.1.13 extracted from
- Manganese peroxidase from Phanerochaete chrysosporium (1988), Methods Enzymol., 161, 258-264.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| alpha-hydroxy acid | stimulates by chelating Mn3+ and stabilizing its high redox potential | Phanerodontia chrysosporium |  |
| citrate | stimulates by chelating Mn3+ and stabilizing its high redox potential | Phanerodontia chrysosporium | |
| H2O2 | H2O2-dependent | Phanerodontia chrysosporium | |
| L-malate | activates by chelating and stabilizing Mn3+ | Phanerodontia chrysosporium | |
| L-Tartrate | - |
Phanerodontia chrysosporium | |
| Lactate | stimulates by complexing with and stabilizing Mn3+ | Phanerodontia chrysosporium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.08 | - |
Mn2+ | - |
Phanerodontia chrysosporium | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Phanerodontia chrysosporium | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
x * 46000, SDS-PAGE | Phanerodontia chrysosporium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Mn2+ + H+ + H2O2 | Phanerodontia chrysosporium | involved in lignin-degradation | Mn3+ + H2O | - |
? | |
| Mn2+ + H+ + H2O2 | Phanerodontia chrysosporium | acts together with lignin peroxidase in lignin-degradation of white rot fungi | Mn3+ + H2O | - |
? | |
| Mn2+ + H+ + H2O2 | Phanerodontia chrysosporium | in absence of H2O2 it may play a role in fungal peroxide production under ligninolytic conditions | Mn3+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phanerodontia chrysosporium | - |
- |
- |
| Phanerodontia chrysosporium OGC101 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 12fold purification | Phanerodontia chrysosporium |
| 3 isozymes | Phanerodontia chrysosporium |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, concentrated preparation, 1 mg/ml, 3 months, stable | Phanerodontia chrysosporium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Phanerodontia chrysosporium | Mn3+ + H2O | product Mn3+ is a nonspecific oxidant which in turn oxidizes a variety of organic compounds | ? | |
| Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes phenolic lignin model compounds | ? | |
| Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes a variety of phenols | ? | |
| Mn2+ + H+ + H2O2 | involved in lignin-degradation | Phanerodontia chrysosporium | Mn3+ + H2O | - |
? | |
| Mn2+ + H+ + H2O2 | acts together with lignin peroxidase in lignin-degradation of white rot fungi | Phanerodontia chrysosporium | Mn3+ + H2O | - |
? | |
| Mn2+ + H+ + H2O2 | in absence of H2O2 it may play a role in fungal peroxide production under ligninolytic conditions | Phanerodontia chrysosporium | Mn3+ + H2O | - |
? | |
| additional information | enzyme oxidizes a variety of organic compounds in presence, but not in absence of Mn2+ | Phanerodontia chrysosporium | ? | - |
? | |
| additional information | enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate | Phanerodontia chrysosporium | ? | - |
? | |
| additional information | in absence of H2O2 the enzyme oxidizes Mn-dependently NADH to NAD+, generating H2O2 for oxidizing other substrates | Phanerodontia chrysosporium | ? | - |
? | |
| additional information | in absence of H2O2 the enzyme oxidizes Mn-dependently NADPH+ to NADP+ | Phanerodontia chrysosporium | ? | - |
? | |
| additional information | no other metal can substitute Mn2+ | Phanerodontia chrysosporium | ? | - |
? | |
| additional information | in presence of Mn2+ enzyme oxidizes various organic compounds | Phanerodontia chrysosporium | ? | - |
? | |
| additional information | enzyme oxidizes a variety of organic compounds in presence, but not in absence of Mn2+ | Phanerodontia chrysosporium OGC101 | ? | - |
? | |
| additional information | enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate | Phanerodontia chrysosporium OGC101 | ? | - |
? | |
| additional information | in absence of H2O2 the enzyme oxidizes Mn-dependently NADH to NAD+, generating H2O2 for oxidizing other substrates | Phanerodontia chrysosporium OGC101 | ? | - |
? | |
| additional information | in absence of H2O2 the enzyme oxidizes Mn-dependently NADPH+ to NADP+ | Phanerodontia chrysosporium OGC101 | ? | - |
? | |
| additional information | no other metal can substitute Mn2+ | Phanerodontia chrysosporium OGC101 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 46000, SDS-PAGE | Phanerodontia chrysosporium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
assay at room temperature | Phanerodontia chrysosporium |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | one iron protoporphyrin IX prosthetic group per enzyme molecule | Phanerodontia chrysosporium | |
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