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Literature summary for 1.11.1.13 extracted from
- Heterogeneity and regulation of manganese peroxidases from Phanerochaete chrysosporium (1992), J. Bacteriol., 174, 3532-3540.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| H2O2 | H2O2-dependent | Phanerodontia chrysosporium |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| isoenzymes H3, H4 and H5 are encoded by different genes and differentially regulated | Phanerodontia chrysosporium |
General Stability
| General Stability | Organism |
|---|---|
| enzyme is more stable in carbon-limited cultures than in nitrogen-limited cultures | Phanerodontia chrysosporium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| H2O2 | inhibitory at high concentrations, phenol red is the most sensitive, vanillylacetone the least sensitive substrate to inhibition, Mn2+ protects against inhibition | Phanerodontia chrysosporium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
Mn2+ | isoenzyme H5 | Phanerodontia chrysosporium | |
| 0.009 | - |
Mn2+ | isoenzyme H4 | Phanerodontia chrysosporium | |
| 0.016 | - |
Mn2+ | isoenzyme H3 | Phanerodontia chrysosporium | |
| 0.021 | - |
H2O2 | isoenzyme H5 | Phanerodontia chrysosporium | |
| 0.022 | - |
vanillylacetone | isoenzyme H5 | Phanerodontia chrysosporium | |
| 0.024 | - |
vanillylacetone | isoenzyme H4 | Phanerodontia chrysosporium | |
| 0.031 | - |
vanillylacetone | isoenzyme H3 | Phanerodontia chrysosporium | |
| 0.032 | - |
H2O2 | isoenzyme H4 | Phanerodontia chrysosporium | |
| 0.034 | - |
H2O2 | isoenzyme H3 | Phanerodontia chrysosporium | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Phanerodontia chrysosporium | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | Mn2+ causes a concentration-dependent increase in total enzyme activity, little or no activity in absence of Mn2+ | Phanerodontia chrysosporium | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Mn2+ + H+ + H2O2 | Phanerodontia chrysosporium | MnP isoenzymes serve different functions in lignin biodegradation, each may have a preferred substrate | Mn3+ + H2O | the product Mn3+ is involved in the oxidative degradation of lignin in white-rot basidiomycetes, induced by Mn2+ | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phanerodontia chrysosporium | - |
- |
- |
| Phanerodontia chrysosporium | - |
white rot basidomycete | - |
| Phanerodontia chrysosporium | - |
isozyme profile depends on type of nutrient limitation and growth phase: no H5 in C-limited cultures, manganese increases H3-production | - |
| Phanerodontia chrysosporium | - |
overproducing strain PSBL-1 | - |
| Phanerodontia chrysosporium | - |
isoenzymes H3, H4 and H5 | - |
| Phanerodontia chrysosporium BKM-F 1767 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity chromatography on blue agarose: isozyme H4 95% pure, followed by preparative isoelectric focusing: isozymes H3 and H5 pure | Phanerodontia chrysosporium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Mn2+ + H+ + H2O2 | little or no enzyme activity in absence of Mn2+ | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes vanillylacetone | ? | |
| Mn2+ + H+ + H2O2 | little or no enzyme activity in absence of Mn2+ | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes phenol red | ? | |
| Mn2+ + H+ + H2O2 | little or no enzyme activity in absence of Mn2+ | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes a variety of phenols | ? | |
| Mn2+ + H+ + H2O2 | little or no enzyme activity in absence of Mn2+ | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes guaiacol | ? | |
| Mn2+ + H+ + H2O2 | MnP isoenzymes serve different functions in lignin biodegradation, each may have a preferred substrate | Phanerodontia chrysosporium | Mn3+ + H2O | the product Mn3+ is involved in the oxidative degradation of lignin in white-rot basidiomycetes, induced by Mn2+ | ? | |
| Mn2+ + H+ + H2O2 | little or no enzyme activity in absence of Mn2+ | Phanerodontia chrysosporium BKM-F 1767 | Mn3+ + H2O | Mn3+ oxidizes vanillylacetone | ? | |
| Mn2+ + H+ + H2O2 | little or no enzyme activity in absence of Mn2+ | Phanerodontia chrysosporium BKM-F 1767 | Mn3+ + H2O | Mn3+ oxidizes phenol red | ? | |
| additional information | enzyme oxidizes phenol red | Phanerodontia chrysosporium | ? | - |
? | |
| additional information | in presence of Mn2+ enzyme oxidizes various organic compounds | Phanerodontia chrysosporium | ? | - |
? | |
| additional information | enzyme oxidizes phenol red | Phanerodontia chrysosporium BKM-F 1767 | ? | - |
? | |
| additional information | in presence of Mn2+ enzyme oxidizes various organic compounds | Phanerodontia chrysosporium BKM-F 1767 | ? | - |
? | |
| phenol red + H2O2 | - |
Phanerodontia chrysosporium | ? | - |
? | |
| phenol red + H2O2 | - |
Phanerodontia chrysosporium BKM-F 1767 | ? | - |
? | |
| vanillylacetone + H2O2 | - |
Phanerodontia chrysosporium | ? | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 39 | - |
Phenol red | isoenzyme H5 | Phanerodontia chrysosporium | |
| 40 | - |
guaiacol | isoenzyme H5 | Phanerodontia chrysosporium | |
| 41 | - |
Phenol red | isoenzyme H4 | Phanerodontia chrysosporium | |
| 53 | - |
guaiacol | isoenzyme H3 | Phanerodontia chrysosporium | |
| 56 | - |
guaiacol | isoenzyme H4 | Phanerodontia chrysosporium | |
| 66 | - |
Phenol red | isoenzyme H3 | Phanerodontia chrysosporium | |
| 76 | - |
vanillylacetone | isoenzyme H5 | Phanerodontia chrysosporium | |
| 90.3 | - |
vanillylacetone | isoenzyme H4 | Phanerodontia chrysosporium | |
| 97 | - |
vanillylacetone | isoenzyme H3 | Phanerodontia chrysosporium | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 4.9 | Phanerodontia chrysosporium |
| additional information | - |
isozyme H5: pI 4.2, isoenzyme H4: pI 4.5, isoenzyme H3: pI 4.9 | Phanerodontia chrysosporium |
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