Cloned (Comment) | Organism |
---|---|
into the vector pET-28a+ for expression in Escherichia coli BL21DE3 cells, into the vector YEpGAP112 for a functional complementation test in yeast | Momordica charantia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01113 | - |
phosphatidylcholine hydroperoxide | apparent KM | Momordica charantia | |
0.6383 | - |
H2O2 | apparent KM | Momordica charantia | |
2.932 | - |
tert-butyl-hydroperoxide | apparent KM | Momordica charantia |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
selenium | - |
Momordica charantia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22500 | - |
His6-McPHGPx fusion protein, determined by SDS-PAGE | Momordica charantia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + a lipid hydroperoxide | Momordica charantia | - |
glutathione disulfide + lipid + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Momordica charantia | Q8W259 | - |
- |
Purification (Comment) | Organism |
---|---|
by nickel affinity chromatography | Momordica charantia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + a lipid hydroperoxide | - |
Momordica charantia | glutathione disulfide + lipid + H2O | - |
? | |
glutathione + H2O2 | - |
Momordica charantia | ? | - |
? | |
glutathione + phosphatidylcholine hydroperoxide | - |
Momordica charantia | ? | - |
? | |
glutathione + tert-butyl-hydroperoxide | - |
Momordica charantia | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Momordica charantia |
Synonyms | Comment | Organism |
---|---|---|
McPHGPx | - |
Momordica charantia |
phospholipid hydroperoxide glutathione peroxidase | - |
Momordica charantia |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
activity assay | Momordica charantia |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | - |
Momordica charantia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
activity assay | Momordica charantia |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 10.5 | - |
Momordica charantia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
glutathione | - |
Momordica charantia |