Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21 Star | Euglena gracilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.493 | - |
L-ascorbate | recombinant full length enzyme, in 50 mM potassium phosphate buffer, pH 7.0, at 22°C | Euglena gracilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | APX becomes mature in the cytosol after processing of the precursor | Euglena gracilis | 5829 | - |
additional information | not localized in plastids | Euglena gracilis | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
2 * 30000, SDS-PAGE | Euglena gracilis |
58000 | - |
mature full length APX, gel filtration | Euglena gracilis |
60080 | - |
mature full length APX, calculated from amino acid sequence | Euglena gracilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Euglena gracilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
TALON metal affinity resin column chromatography | Euglena gracilis |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-isoascorbate + H2O2 | 60.3% activity compared to L-ascorbate | Euglena gracilis | ? | - |
? | |
L-ascorbate + cumene hydroperoxide | 8.0% activity compared to H2O2 | Euglena gracilis | ? | - |
? | |
L-ascorbate + H2O2 | 100% activity | Euglena gracilis | dehydroascorbate + H2O | - |
? | |
L-ascorbate + tert-butyl hydroperoxide | 17.4% activity compared to H2O2 | Euglena gracilis | ? | - |
? | |
additional information | no activity with guaiacol and NADPH | Euglena gracilis | ? | - |
? | |
pyrogallol + H2O2 | 90.1% activity compared to L-ascorbate | Euglena gracilis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 30000, SDS-PAGE | Euglena gracilis |
Synonyms | Comment | Organism |
---|---|---|
APX | - |
Euglena gracilis |
ascorbate peroxidase | - |
Euglena gracilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
577 | - |
H2O2 | recombinant full length enzyme, in 50 mM potassium phosphate buffer, pH 7.0, at 22°C | Euglena gracilis | |
754 | - |
L-ascorbate | recombinant full length enzyme, in 50 mM potassium phosphate buffer, pH 7.0, at 22°C | Euglena gracilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Euglena gracilis |
General Information | Comment | Organism |
---|---|---|
physiological function | APX is physiologically important to the metabolism of H2O2 | Euglena gracilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1530 | - |
L-ascorbate | recombinant full length enzyme, in 50 mM potassium phosphate buffer, pH 7.0, at 22°C | Euglena gracilis | |
16500 | - |
H2O2 | recombinant full length enzyme, in 50 mM potassium phosphate buffer, pH 7.0, at 22°C | Euglena gracilis |