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Literature summary for 1.11.1.11 extracted from

  • Ishikawa, T.; Tajima, N.; Nishikawa, H.; Gao, Y.; Rapolu, M.; Shibata, H.; Sawa, Y.; Shigeoka, S.
    Euglena gracilis ascorbate peroxidase forms an intramolecular dimeric structure: its unique molecular characterization (2010), Biochem. J., 426, 125-134.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21 Star Euglena gracilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.493
-
L-ascorbate recombinant full length enzyme, in 50 mM potassium phosphate buffer, pH 7.0, at 22°C Euglena gracilis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol APX becomes mature in the cytosol after processing of the precursor Euglena gracilis 5829
-
additional information not localized in plastids Euglena gracilis
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
2 * 30000, SDS-PAGE Euglena gracilis
58000
-
mature full length APX, gel filtration Euglena gracilis
60080
-
mature full length APX, calculated from amino acid sequence Euglena gracilis

Organism

Organism UniProt Comment Textmining
Euglena gracilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
TALON metal affinity resin column chromatography Euglena gracilis

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-isoascorbate + H2O2 60.3% activity compared to L-ascorbate Euglena gracilis ?
-
?
L-ascorbate + cumene hydroperoxide 8.0% activity compared to H2O2 Euglena gracilis ?
-
?
L-ascorbate + H2O2 100% activity Euglena gracilis dehydroascorbate + H2O
-
?
L-ascorbate + tert-butyl hydroperoxide 17.4% activity compared to H2O2 Euglena gracilis ?
-
?
additional information no activity with guaiacol and NADPH Euglena gracilis ?
-
?
pyrogallol + H2O2 90.1% activity compared to L-ascorbate Euglena gracilis ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 30000, SDS-PAGE Euglena gracilis

Synonyms

Synonyms Comment Organism
APX
-
Euglena gracilis
ascorbate peroxidase
-
Euglena gracilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
577
-
H2O2 recombinant full length enzyme, in 50 mM potassium phosphate buffer, pH 7.0, at 22°C Euglena gracilis
754
-
L-ascorbate recombinant full length enzyme, in 50 mM potassium phosphate buffer, pH 7.0, at 22°C Euglena gracilis

Cofactor

Cofactor Comment Organism Structure
heme
-
Euglena gracilis

General Information

General Information Comment Organism
physiological function APX is physiologically important to the metabolism of H2O2 Euglena gracilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1530
-
L-ascorbate recombinant full length enzyme, in 50 mM potassium phosphate buffer, pH 7.0, at 22°C Euglena gracilis
16500
-
H2O2 recombinant full length enzyme, in 50 mM potassium phosphate buffer, pH 7.0, at 22°C Euglena gracilis