Any feedback?
Literature summary for 1.11.1.11 extracted from
- Expression of the Cyanidioschyzon merolae stromal ascorbate peroxidase in Arabidopsis thaliana enhances thermotolerance (2009), Plant Cell Rep., 28, 1881-1893.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| full length AtstAPX is cloned into the pPZP221 binary vector, introduced into Arabidopsis thaliana by the floral dip method using Agrobacterium tumefaciens strain GV3101, for the generation of antibodies into the vector pQE80L for expression in Escherichia coli cells | Arabidopsis thaliana |
| full length CmstAPX is cloned into the pPZP221 binary vector, introduced into Arabidopsis thaliana by the floral dip method using Agrobacterium tumefaciens strain GV3101, for the generation of antibodies into the vector pQE80L for expression in Escherichia coli cells | Cyanidioschyzon merolae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Arabidopsis thaliana | 9507 | - |
| chloroplast | - |
Cyanidioschyzon merolae | 9507 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
determined by SDS-PAGE and Western Blot analysis | Arabidopsis thaliana |
| 30000 | - |
determined by SDS-PAGE and Western Blot analysis | Cyanidioschyzon merolae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-ascorbate + H2O2 | Arabidopsis thaliana | - |
dehydroascorbate + H2O | - |
? |  |
| L-ascorbate + H2O2 | Cyanidioschyzon merolae | - |
dehydroascorbate + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
| Cyanidioschyzon merolae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Escherichia coli recombinant stromal ascorbate peroxidase is purified with a HisTrap kit, proteins are used to immunize rabbits and rats | Arabidopsis thaliana |
| Escherichia coli recombinant stromal ascorbate peroxidase is purified with a HisTrap kit, proteins are used to immunize rabbits and rats | Cyanidioschyzon merolae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Arabidopsis thaliana | - |
| leaf | - |
Cyanidioschyzon merolae | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-ascorbate + H2O2 | - |
Arabidopsis thaliana | dehydroascorbate + H2O | - |
? | |
| L-ascorbate + H2O2 | - |
Cyanidioschyzon merolae | dehydroascorbate + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ascorbate peroxidase | - |
Arabidopsis thaliana |
| ascorbate peroxidase | - |
Cyanidioschyzon merolae |
| AtstAPX | - |
Arabidopsis thaliana |
| CmstAPX | - |
Cyanidioschyzon merolae |
| stromal ascorbate peroxidase | - |
Arabidopsis thaliana |
| stromal ascorbate peroxidase | - |
Cyanidioschyzon merolae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
activity assay | Arabidopsis thaliana |
| 7 | - |
activity assay | Cyanidioschyzon merolae |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
