Protein Variants | Comment | Organism |
---|---|---|
C25S | kcat/Km for L-ascorbate is 5.4fold lower than wild-type value. kcat/KM for H2O2 is 2.1fold lower than wild-type value. In contrast to wild-type enzyme, the mutant enzyme retains more than 90% of the initial activity after incubation for 10 min with the radical scavenger 2,2,6,6-tetramethylpiperidinyl-1-oxy and H2O2 | Galdieria partita |
C25S/C121S | kcat/Km for L-ascorbate is 4.5fold lower than wild-type value. kcat/KM for H2O2 is 2.1fold lower than wild-type value. In contrast to wild-type enzyme, the mutant enzyme retains more than 90% of the initial activity after incubation for 10 min with the radical scavenger 2,2,6,6-tetramethylpiperidinyl-1-oxy and H2O2 | Galdieria partita |
C26S | kcat/Km for L-ascorbate is 1.8fold lower than wild-type value. kcat/KM for H2O2 is 3.1fold lower than wild-type value. In contrast to wild-type enzyme, the mutant enzyme retains 60% of the initial activity after incubation for 10 min with the radical scavenger 2,2,6,6-tetramethylpiperidinyl-1-oxy and H2O2 | Nicotiana tabacum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2,6,6-tetramethylpiperidinyl-1-oxide | formation of 2,2,6,6-tetramethylpiperidinyl-1-oxy-adducts and subsequent oxidation of the cysteine residue located near the propionate group of heme leads to loss of enzyme activity | Nicotiana tabacum | |
2,2,6,6-tetramethylpiperidinyl-1-oxyl radical | formation of 2,2,6,6-tetramethylpiperidinyl-1-oxy-adducts and subsequent oxidation of the cysteine residue located near the propionate group of heme leads to loss of enzyme activity | Galdieria partita |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
H2O2 | wild-type enzyme | Nicotiana tabacum | |
0.042 | - |
H2O2 | wild-type enzyme | Galdieria partita | |
0.05 | - |
H2O2 | mutant enzyme C25S | Galdieria partita | |
0.052 | - |
H2O2 | mutant enzyme C25S/C121S | Galdieria partita | |
0.061 | - |
H2O2 | mutant enzyme C26S | Nicotiana tabacum | |
0.117 | - |
L-ascorbate | wild-type enzyme | Galdieria partita | |
0.394 | - |
L-ascorbate | mutant enzyme C25S/C121S | Galdieria partita | |
0.395 | - |
L-ascorbate | wild-type enzyme | Nicotiana tabacum | |
0.496 | - |
L-ascorbate | mutant enzyme C25S | Galdieria partita | |
0.566 | - |
L-ascorbate | mutant enzyme C26S | Nicotiana tabacum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | stroma | Nicotiana tabacum | 9507 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Galdieria partita | - |
- |
- |
Nicotiana tabacum | Q9TNL9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ascorbate + H2O2 | - |
Galdieria partita | dehydroascorbate + H2O | - |
? | |
L-ascorbate + H2O2 | - |
Nicotiana tabacum | dehydroascorbate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
APX | - |
Nicotiana tabacum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1230 | - |
H2O2 | mutant enzyme C25S | Galdieria partita | |
1310 | - |
H2O2 | mutant enzyme C25S/C121S | Galdieria partita | |
1490 | - |
L-ascorbate | mutant enzyme C25S/C121S | Galdieria partita | |
1570 | - |
L-ascorbate | mutant enzyme C25S | Galdieria partita | |
1580 | - |
H2O2 | mutant enzyme C26S | Nicotiana tabacum | |
1800 | - |
H2O2 | wild-type enzyme | Nicotiana tabacum | |
1980 | - |
L-ascorbate | wild-type enzyme | Galdieria partita | |
2040 | - |
L-ascorbate | mutant enzyme C26S | Nicotiana tabacum | |
2190 | - |
H2O2 | wild-type enzyme | Galdieria partita | |
2510 | - |
L-ascorbate | wild-type enzyme | Nicotiana tabacum |