Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli of two soybean ascorbate peroxidase cDNAs | Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
C2H2 | recombinant enzyme 1: 94% inhibition at 0.1 ml per l, recombinant enzyme 2: 2% inhibition at 0.1 ml per l | Glycine max | |
hydroxylamine | recombinant enzyme 1: 74% inhibition at 1 mM and 100% inhibition at 10 mM, recombinant enzyme 2: 86% inhibition at 1 mM and 100% inhibition at 10 mM | Glycine max | |
KCN | recombinant enzyme 1: 69% inhibition at 0.1 mM and 100% inhibition at 0.5 mM, recombinant enzyme 2: 81% inhibition at 0.1 mM and 100% inhibition at 0.5 mM | Glycine max | |
p-chloromercuriphenyl sulfonic acid | 100% inhibition at 0.05 mM, recombinant enzyme 1 and 2 | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
H2O2 | recombinant enzyme 1 | Glycine max | |
0.024 | - |
H2O2 | recombinant enzyme 2 | Glycine max | |
0.333 | - |
ascorbate | recombinant enzyme 1 | Glycine max | |
0.467 | - |
ascorbate | recombinant enzyme 2 | Glycine max |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | presence of two major non-plastid isozymes | Glycine max | 5829 | - |
additional information | the doublet in cytosolic enzyme activity observed in the native-PAGE of soybean leaf and root nodule extracts could represent either the products of two cytosolic soybean enzyme genes or a single gene product with some post-translational modification, the cultivar Roanoke has a single isozyme | Glycine max | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
recombinant enzyme 1 and 2, calculated from sequence of cDNA | Glycine max |
28000 | - |
x * 28000, SDS-PAGE, recombinant enzyme 2 | Glycine max |
30000 | - |
x * 30000, SDS-PAGE, recombinant enzyme 1 | Glycine max |
46000 | - |
recombinant enzyme 2, column chromatography in the absence of denaturant | Glycine max |
48000 | - |
recombinant enzyme 1, column chromatography in the absence of denaturant | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
soybean | - |
Purification (Comment) | Organism |
---|---|
of recombinant enzyme 1: using column chromatography on DEAE Bio-Gel A agarose and column chromatography on Sephacryl S-300, of recombinant enzyme 2: using column chromatography on DEAE Bio-Gel A agarose, ammonium sulfate fractionation, dialysis, chromatofocusing on a polybuffer 94 exchanger and column chromatography on Sephacryl S-300 | Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hypocotyl | presence of two major non-plastid isozymes | Glycine max | - |
leaf | presence of two major non-plastid isozymes | Glycine max | - |
root | nodules | Glycine max | - |
root | presence of two major non-plastid isozymes | Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.4 | - |
recombinant enzyme 1, after DEAE-column chromatography | Glycine max |
63.6 | - |
recombinant enzyme 2, after chromatofocusing | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guaiacol + H2O2 | recombinant enzyme 1: 6% activity relative to L-ascorbate, recombinant enzyme 2: 11% relative activity to L-ascorbate | Glycine max | ? | - |
? | |
L-ascorbate + H2O2 | - |
Glycine max | dehydroascorbate + H2O | - |
? | |
pyrogallol + H2O2 | recombinant enzyme 1: 355% activity relative to L-ascorbate, recombinant enzyme 2: 304% activity relative to L-ascorbate | Glycine max | 3-hydroxybenzo-1,2-quinone + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, SDS-PAGE, recombinant enzyme 1 | Glycine max |
? | x * 28000, SDS-PAGE, recombinant enzyme 2 | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
recombinant enzyme 2 | Glycine max |
7 | - |
assay at | Glycine max |
7 | - |
recombinant enzyme 1 | Glycine max |