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Literature summary for 1.11.1.11 extracted from

  • Caldwell, C.R.; Turano, F.J.; McMahon, M.B.
    Identification of two cytosolic ascorbate peroxidase cDNAs from soybean leaves and characterization of their products by functional expression in E. coli (1998), Planta, 204, 120-126.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli of two soybean ascorbate peroxidase cDNAs Glycine max

Inhibitors

Inhibitors Comment Organism Structure
C2H2 recombinant enzyme 1: 94% inhibition at 0.1 ml per l, recombinant enzyme 2: 2% inhibition at 0.1 ml per l Glycine max
hydroxylamine recombinant enzyme 1: 74% inhibition at 1 mM and 100% inhibition at 10 mM, recombinant enzyme 2: 86% inhibition at 1 mM and 100% inhibition at 10 mM Glycine max
KCN recombinant enzyme 1: 69% inhibition at 0.1 mM and 100% inhibition at 0.5 mM, recombinant enzyme 2: 81% inhibition at 0.1 mM and 100% inhibition at 0.5 mM Glycine max
p-chloromercuriphenyl sulfonic acid 100% inhibition at 0.05 mM, recombinant enzyme 1 and 2 Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
H2O2 recombinant enzyme 1 Glycine max
0.024
-
H2O2 recombinant enzyme 2 Glycine max
0.333
-
ascorbate recombinant enzyme 1 Glycine max
0.467
-
ascorbate recombinant enzyme 2 Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol presence of two major non-plastid isozymes Glycine max 5829
-
additional information the doublet in cytosolic enzyme activity observed in the native-PAGE of soybean leaf and root nodule extracts could represent either the products of two cytosolic soybean enzyme genes or a single gene product with some post-translational modification, the cultivar Roanoke has a single isozyme Glycine max
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
recombinant enzyme 1 and 2, calculated from sequence of cDNA Glycine max
28000
-
x * 28000, SDS-PAGE, recombinant enzyme 2 Glycine max
30000
-
x * 30000, SDS-PAGE, recombinant enzyme 1 Glycine max
46000
-
recombinant enzyme 2, column chromatography in the absence of denaturant Glycine max
48000
-
recombinant enzyme 1, column chromatography in the absence of denaturant Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
soybean
-

Purification (Commentary)

Purification (Comment) Organism
of recombinant enzyme 1: using column chromatography on DEAE Bio-Gel A agarose and column chromatography on Sephacryl S-300, of recombinant enzyme 2: using column chromatography on DEAE Bio-Gel A agarose, ammonium sulfate fractionation, dialysis, chromatofocusing on a polybuffer 94 exchanger and column chromatography on Sephacryl S-300 Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
hypocotyl presence of two major non-plastid isozymes Glycine max
-
leaf presence of two major non-plastid isozymes Glycine max
-
root nodules Glycine max
-
root presence of two major non-plastid isozymes Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.4
-
recombinant enzyme 1, after DEAE-column chromatography Glycine max
63.6
-
recombinant enzyme 2, after chromatofocusing Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guaiacol + H2O2 recombinant enzyme 1: 6% activity relative to L-ascorbate, recombinant enzyme 2: 11% relative activity to L-ascorbate Glycine max ?
-
?
L-ascorbate + H2O2
-
Glycine max dehydroascorbate + H2O
-
?
pyrogallol + H2O2 recombinant enzyme 1: 355% activity relative to L-ascorbate, recombinant enzyme 2: 304% activity relative to L-ascorbate Glycine max 3-hydroxybenzo-1,2-quinone + H2O
-
?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE, recombinant enzyme 1 Glycine max
? x * 28000, SDS-PAGE, recombinant enzyme 2 Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6
-
recombinant enzyme 2 Glycine max
7
-
assay at Glycine max
7
-
recombinant enzyme 1 Glycine max