Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.11.1.10 extracted from

  • Yadav, P.; Yadav, M.; Yadav, K.; Sharma, J.; Singh, V.
    Purification of chloroperoxidase from Musa paradisiaca stem juice (2013), Int. J. Chem. Kinetics, 45, 92-100.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
azide
-
Musa x paradisiaca

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
monochlorodimedone in 50 mM phosphate buffer pH 2.75 at 30°C, with 20 mM KCl Musa x paradisiaca
0.089
-
pyrogallol in 50 mM phosphate buffer pH 2.75 at 30°C, with 20 mM KCl Musa x paradisiaca
0.12
-
H2O2 in 50 mM phosphate buffer pH 2.75 at 30°C, with 20 mM KCl Musa x paradisiaca

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
x * 43000, SDS-PAGE Musa x paradisiaca

Organism

Organism UniProt Comment Textmining
Musa x paradisiaca
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ultrafiltration and DEAE cellulose column chromatography Musa x paradisiaca

Source Tissue

Source Tissue Comment Organism Textmining
stem juice
-
Musa x paradisiaca
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.27
-
crude enzyme, at pH 2.75 and 30°C Musa x paradisiaca
2.25
-
after 8.33fold purification, at pH 2.75 and 30°C Musa x paradisiaca

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-chloroaniline + chloride + H2O2
-
Musa x paradisiaca ?
-
?
monochlorodimedone + chloride + H2O2
-
Musa x paradisiaca dichlorodimedone + H2O
-
?
pyrogallol + chloride + H2O2
-
Musa x paradisiaca ?
-
?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Musa x paradisiaca

Synonyms

Synonyms Comment Organism
chloroperoxidase
-
Musa x paradisiaca
CPO
-
Musa x paradisiaca

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
Musa x paradisiaca

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.64
-
monochlorodimedone in 50 mM phosphate buffer pH 2.75 at 30°C, with 20 mM KCl Musa x paradisiaca
26.56
-
pyrogallol in 50 mM phosphate buffer pH 2.75 at 30°C, with 20 mM KCl Musa x paradisiaca

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
-
Musa x paradisiaca

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.5 6 at pH 2.75 it loses 85% activity in 1 h, and it is completely inactivated in 24 h. At pH 4.4, the enzyme also loses activity with time and is completely inactivated after 2.5 h. However, the enzyme exposed to pH 6.0 has low activity but it remains constant for 24 h Musa x paradisiaca

Cofactor

Cofactor Comment Organism Structure
heme
-
Musa x paradisiaca

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
82
-
monochlorodimedone in 50 mM phosphate buffer pH 2.75 at 30°C, with 20 mM KCl Musa x paradisiaca
298.4
-
pyrogallol in 50 mM phosphate buffer pH 2.75 at 30°C, with 20 mM KCl Musa x paradisiaca