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Literature summary for 1.11.1.10 extracted from

  • Grey, C.E.; Rundbaeck, F.; Adlercreutz, P.
    Improved operational stability of chloroperoxidase through use of antioxidants (2008), J. Biotechnol., 135, 196-201.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ascorbic acid enhances total turnover number compared to reaction without antioxidant Leptoxyphium fumago
caffeic acid the total turnover number is almost 5fold higher than that obtained for the reference reaction Leptoxyphium fumago
citraconic anhydride the catalytic efficiency of the modified enzyme for sulfoxidation in aqueous buffer is increased by 26.2% Leptoxyphium fumago
ferulic acid enhances total turnover number compared to reaction without antioxidant Leptoxyphium fumago
Maleic anhydride the catalytic efficiency of the modified enzyme for sulfoxidation in aqueous buffer is increased by 22.6% Leptoxyphium fumago
phthalic anhydride the catalytic efficiency of the modified enzyme for sulfoxidation in aqueous buffer is increased by 12.9% Leptoxyphium fumago

General Stability

General Stability Organism
the thermostability of peroxidase of CPO is increased about 2fold upon these chemical modification by citraconic anhydride, phthalic anhydride or maleic anhydride. The thermostability of sulfoxidation activity of CPO is increased about 1.2fold upon the chemical modification by citraconic anhydride, phthalic anhydride or maleic anhydride Leptoxyphium fumago

Organism

Organism UniProt Comment Textmining
Leptoxyphium fumago
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
indole + H2O2
-
Leptoxyphium fumago 2-oxoindole + H2O
-
?
indole + tert-butyl hydroperoxide
-
Leptoxyphium fumago 2-oxoindole + ?
-
?

Synonyms

Synonyms Comment Organism
chloroperoxidase
-
Leptoxyphium fumago
CPO
-
Leptoxyphium fumago

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
the thermostability of peroxidase of CPO is increased about 2fold upon these chemical modification by citraconic anhydride, phthalic anhydride or maleic anhydride. The thermostability of sulfoxidation activity of CPO is increased about 1.2fold upon these chemical modification by citraconic anhydride, phthalic anhydride or maleic anhydride Leptoxyphium fumago
50
-
6 h, native CPO retains about 10% peroxidase activity and 20% sulfoxidation activity in aqueous buffer. CPOs modified by citraconic anhydride, phthalic anhydride or maleic anhydride, retain about 30% peroxidase activity and 30% sulfoxidation activity Leptoxyphium fumago