General Stability | Organism |
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PEG200 and glycerol are the most efficient stabilizer for CPO in temperatures ranging from 25°C to 60°C. Trehalose is more helpful than other sugars for extended storage of CPO | Leptoxyphium fumago |
Organism | UniProt | Comment | Textmining |
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Leptoxyphium fumago | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
indole + H2O2 | - |
Leptoxyphium fumago | 2-oxoindole + H2O | - |
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Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
PEG200 and glycerol are the most efficient stabilizer for CPO in temperatures ranging from 25°C to 60°C | Leptoxyphium fumago |
25 | - |
the activity of CPO is almost demolished in the presence of 0.01 M glucose after only 9 h. Dioctyl sulfosuccinate sodium salt, PEG200, and glycerol exhibit strong stabilizing effect and are capable of preventing CPO from inactivation in the 432 h incubation period | Leptoxyphium fumago |
40 | - |
the stability of CPO is decreased by glucose, fructose, galactose, xylose, dextran, and surfactant dioctyl sulfosuccinate sodium salt. Stability can be increased by PEG200, glycerol, trehalose, and gum sugar. In the absence of the additives, CPO loses almost all of its activity after 90 h. However, in PEG200 solutions, 88.5% activity is retained after 329 h. The storage time of CPO in trehalose and gum sugar can be prolonged to 233 and 113 h, respectively | Leptoxyphium fumago |
50 | - |
the native enzyme loses almost all of its activity after 10 h of incubation, in 0.2 M glycerol the enzyme retains activity for up to 200 h. In the presence of 0.05 M PEG200, the enzyme remains as active as freshly prepared samples in the first 10 h. After 192 h of incubation, 13% activity could still be retained. Enzyme retains 24% of its activity after 24 h of standing in dextran-containing buffers | Leptoxyphium fumago |
60 | - |
activity of CPO is almost completely lost in the first 50 min of incubation in the absence of additives. In the presence of PEG200, trehalose, galactose, glycerol, gum sugar, and dextran, 26.5%, 17.7%, 15.6%, 12.4%, 2.6%, and 1.7%, respectively, of enzyme activity can be rescued | Leptoxyphium fumago |