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Literature summary for 1.11.1.10 extracted from

  • Zhi, L.; Jiang, Y.; Wang, Y.; Hu, M.; Li, S.; Ma, Y.
    Effects of additives on the thermostability of chloroperoxidase (2007), Biotechnol. Prog., 23, 729-733.
    View publication on PubMed

General Stability

General Stability Organism
PEG200 and glycerol are the most efficient stabilizer for CPO in temperatures ranging from 25°C to 60°C. Trehalose is more helpful than other sugars for extended storage of CPO Leptoxyphium fumago

Organism

Organism UniProt Comment Textmining
Leptoxyphium fumago
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
indole + H2O2
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Leptoxyphium fumago 2-oxoindole + H2O
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?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
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PEG200 and glycerol are the most efficient stabilizer for CPO in temperatures ranging from 25°C to 60°C Leptoxyphium fumago
25
-
the activity of CPO is almost demolished in the presence of 0.01 M glucose after only 9 h. Dioctyl sulfosuccinate sodium salt, PEG200, and glycerol exhibit strong stabilizing effect and are capable of preventing CPO from inactivation in the 432 h incubation period Leptoxyphium fumago
40
-
the stability of CPO is decreased by glucose, fructose, galactose, xylose, dextran, and surfactant dioctyl sulfosuccinate sodium salt. Stability can be increased by PEG200, glycerol, trehalose, and gum sugar. In the absence of the additives, CPO loses almost all of its activity after 90 h. However, in PEG200 solutions, 88.5% activity is retained after 329 h. The storage time of CPO in trehalose and gum sugar can be prolonged to 233 and 113 h, respectively Leptoxyphium fumago
50
-
the native enzyme loses almost all of its activity after 10 h of incubation, in 0.2 M glycerol the enzyme retains activity for up to 200 h. In the presence of 0.05 M PEG200, the enzyme remains as active as freshly prepared samples in the first 10 h. After 192 h of incubation, 13% activity could still be retained. Enzyme retains 24% of its activity after 24 h of standing in dextran-containing buffers Leptoxyphium fumago
60
-
activity of CPO is almost completely lost in the first 50 min of incubation in the absence of additives. In the presence of PEG200, trehalose, galactose, glycerol, gum sugar, and dextran, 26.5%, 17.7%, 15.6%, 12.4%, 2.6%, and 1.7%, respectively, of enzyme activity can be rescued Leptoxyphium fumago