Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 98% inhibition at 0.01 mM | Enterococcus faecalis | |
Cl- | strong inhibitor | Enterococcus faecalis | |
Co2+ | 25% inhibition at 1 mM | Enterococcus faecalis | |
Cu2+ | 85% inhibition at 1 mM | Enterococcus faecalis | |
H2O2 | 43% inhibition at 16.5 mM | Enterococcus faecalis | |
Hg2+ | 21% inhibition at 0.01 mM | Enterococcus faecalis | |
NaN3 | 15% inhibition at 50 mM | Enterococcus faecalis | |
p-chloromercuribenzoate | 20% inhibition at 0.33 mM | Enterococcus faecalis | |
Pb2+ | 49% inhibition at 0.33 mM | Enterococcus faecalis | |
SO42- | strong inhibitor | Enterococcus faecalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
ferricyanide | - |
Enterococcus faecalis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | small amounts, significance unknown | Enterococcus faecalis | |
additional information | no Mo, Cu, Mn, Zn | Enterococcus faecalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
calculation from FAD content | Enterococcus faecalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + H2O2 | Enterococcus faecalis | - |
NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Enterococcus faecalis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
8500-9000 U/ml, calculated as change of 0.01 of optical density | Enterococcus faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ferricyanide + H2O2 | - |
Enterococcus faecalis | ferrocyanide + H2O | - |
? | |
additional information | NADH cannot be replaced by reduced cytochrome c, H2O2 cannot be replaced by Cu2+, Fe3+, SeO32+, NO3-, NO2-, oxidized glutathione, cystine, lipoic acid disulfide, dehydroascorbic acid, cytochrome c, FAD, flavin mononucleotide, riboflavin, 2,6-dichlorophenolindophenol, methylene blue, brilliant cresyl blue | Enterococcus faecalis | ? | - |
? | |
NADH + H2O2 | - |
Enterococcus faecalis | NAD+ + H2O | - |
? | |
NADH + H2O2 | H2O2 can be replaced by 1,4-naphthoquinone which is one third as effective as H2O2 | Enterococcus faecalis | NAD+ + H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
26 | - |
- |
Enterococcus faecalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
83.3 | - |
NADH | under standard assay conditions | Enterococcus faecalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
ferricyanide | Enterococcus faecalis |
5.2 | 5.4 | substrate H2O2 in acetate or phosphate buffer | Enterococcus faecalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | FAD-flavoprotein, 1 mol FAD per 1 mol enzyme | Enterococcus faecalis |