Cloned (Comment) | Organism |
---|---|
expression of His-tagged OR2 in Spodoptera frugiperda Sf9 cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dicoumarol | - |
Homo sapiens | |
Melatonin | - |
Homo sapiens | |
resveratrol | - |
Homo sapiens | |
S29434 | synthetic compound, complete inhibition | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(beta-D-ribofuranosyl)-1,4-dihydronicotinamide + co-enzyme Q | Homo sapiens | natural substrate nicotinamide riboside, NRH | 1-(beta-D-ribofuranosyl)nicotinamide + reduced co-enzyme Q | - |
? | |
1-(beta-D-ribofuranosyl)-1,4-dihydronicotinamide + menadione | Homo sapiens | natural substrate nicotinamide riboside, NRH | 1-(beta-D-ribofuranosyl)nicotinamide + menadiol | - |
? | |
additional information | Homo sapiens | the enzyme catalyzes the reduction of quinones, such as menadione and co-enzyme Q | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged OR2 from Spodoptera frugiperda Sf9 cells by nickel affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(beta-D-ribofuranosyl)-1,4-dihydronicotinamide + a quinone | - |
Homo sapiens | 1-(beta-D-ribofuranosyl)nicotinamide + a hydroquinone | - |
? | |
1-(beta-D-ribofuranosyl)-1,4-dihydronicotinamide + co-enzyme Q | natural substrate nicotinamide riboside, NRH | Homo sapiens | 1-(beta-D-ribofuranosyl)nicotinamide + reduced co-enzyme Q | - |
? | |
1-(beta-D-ribofuranosyl)-1,4-dihydronicotinamide + menadione | natural substrate nicotinamide riboside, NRH | Homo sapiens | 1-(beta-D-ribofuranosyl)nicotinamide + menadiol | - |
? | |
additional information | the enzyme catalyzes the reduction of quinones, such as menadione and co-enzyme Q | Homo sapiens | ? | - |
? | |
additional information | QR2 produces free radicals with pro-drug CB1954, i.e. 5-(aziridin-1-yl)-2,4-dinitrobenzamide. The anti-cancer pro-drug CB1954 (5-(aziridin-1-yl)-2,4-dinitrobenzamide) is transformed into a potent cytotoxic drug upon reduction of its 4-nitro group to a 4-hydroxylamine by quinone reductases or nitroreductase | Homo sapiens | ? | - |
? | |
N-benzyldihydronicotinamide + a quinone | synthetic substrate | Homo sapiens | N-benzylnicotinamide + a hydroquinone | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | the enzyme forms homodimers with two independent and equivalent active sites each containing a FAD cofactor | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
NRH:QR2 | - |
Homo sapiens |
QR2 | - |
Homo sapiens |
quinone reductase 2 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1 molecule per subunit, binds to the active site of each subunit | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | comparison of isoenzymes Qr1 and QR2, overview | Homo sapiens |