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Literature summary for 1.10.3.4 extracted from

  • Rao, P.V.S.; Vaidyanathan, C.S.
    Studies on the metabolism of o-aminophenol. Purification and properties of isophenoxazine synthase from Bauhenia monandra (1967), Arch. Biochem. Biophys., 118, 388-394.
    View publication on PubMed

General Stability

General Stability Organism
stable to extensive dialysis against cyanide or EDTA Bauhinia monandra

Inhibitors

Inhibitors Comment Organism Structure
2,3-Dimercaptopropanol
-
Bauhinia monandra
3-hydroxyanthranilic acid competitive Bauhinia monandra
Ag+
-
Bauhinia monandra
anthranilic acid competitive Bauhinia monandra
ascorbic acid
-
Bauhinia monandra
Atebrin slightly inhibitory Bauhinia monandra
azide
-
Bauhinia monandra
Cu2+
-
Bauhinia monandra
cyanide
-
Bauhinia monandra
cysteine
-
Bauhinia monandra
Fe2+
-
Bauhinia monandra
glutathione
-
Bauhinia monandra
Hg2+
-
Bauhinia monandra
N-ethylmaleimide no effect Bauhinia monandra
p-hydroxymercuribenzoate reversed by glutathione or cysteine Bauhinia monandra

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.75
-
o-aminophenol
-
Bauhinia monandra

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metal requirement Bauhinia monandra

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bauhinia monandra involved in biosynthesis of catechol ?
-
?

Organism

Organism UniProt Comment Textmining
Bauhinia monandra
-
leguminous plant
-

Purification (Commentary)

Purification (Comment) Organism
-
Bauhinia monandra

Reaction

Reaction Comment Organism Reaction ID
4 2-aminophenol + 3 O2 = 2 2-aminophenoxazin-3-one + 6 H2O discussion of mechanism, isophenoxazine may be formed by a secondary condensation from the initial oxidation product Bauhinia monandra

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
56.1
-
-
Bauhinia monandra

Storage Stability

Storage Stability Organism
-20°C, stable for at least 1 month Bauhinia monandra

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 2-aminophenol + 2 O2 highly specific for o-aminophenol, not: related compounds (e.g. 3-hydroxyanthranilic acid, 3-hydroxy-kynurenine, p-aminophenol, catechol) Bauhinia monandra isophenoxazine + 2 H2O + H2O2
-
?
additional information involved in biosynthesis of catechol Bauhinia monandra ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Bauhinia monandra

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
-
Bauhinia monandra

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor requirement Bauhinia monandra