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Literature summary for 1.10.3.2 extracted from

  • Huang, W.; Tai, R.; Hseu, R.; Huang, C.
    Overexpression and characterization of a thermostable, pH-stable and organic solvent-tolerant Ganoderma fornicatum laccase in Pichia pastoris (2011), Process Biochem., 46, 1469-1474.
No PubMed abstract available
Search for more literature for 1.10.3.2

Cloned(Commentary)

Cloned (Comment) Organism
gene lac1, DNA and amino acid sequence determination and analysis, sequence comparisons, overexpression in Pichia pastoris and Escherichia coli strain DH5aalpha Ganoderma fornicatum

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by EDTA Ganoderma fornicatum
Sodium azide strong inhibition Ganoderma fornicatum
thioglycolic acid strong inhibition Ganoderma fornicatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.104
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) recombinant enzyme, pH 3.0, 30°C Ganoderma fornicatum
0.277
-
 2,6-dimethoxyphenol recombinant enzyme, pH 3.0, 30°C Ganoderma fornicatum
1.263
-
 guaiacol recombinant enzyme, pH 3.0, 30°C Ganoderma fornicatum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54100
-
 x + 63700, glycosylated recombinant enzyme, SDS-PAGE, x * 54100, about, sequence calculation Ganoderma fornicatum

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone rLac1 retains less than 20% activity in 50% v/v acetone after a 3 h incubation at 25°C Ganoderma fornicatum
acetonitril rLac1 retains less than 20% activity in 50% v/v acetonitril after a 3 h incubation at 25°C Ganoderma fornicatum
dimethylformamide rLac1 retains more than 80% activity in 50% v/v dimethylformamide after a 3 h incubation at 25°C Ganoderma fornicatum
DMSO rLac1 retains more than 80% activity in 50% v/v DMSO after a 3 h incubation at 25°C Ganoderma fornicatum
Ethanol rLac1 retains more than 80% activity in 50% v/v ethanol after a 3 h incubation at 25°C Ganoderma fornicatum
Methanol rLac1 retains more than 80% activity in 50% v/v methanol after a 3 h incubation at 25°C Ganoderma fornicatum

Organism

Organism UniProt Comment Textmining
Ganoderma fornicatum B5G555 gene lac1
-
Ganoderma fornicatum 0814 B5G555 gene lac1
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein deglycoslation with endoglycosylase H Ganoderma fornicatum

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme 3.8fold by ulrafiltration, anion exchange chromatography, again ultrafiltration, and gel filtration Ganoderma fornicatum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
59.27
-
 purified recombinant enzyme, pH 3.0, 30°C Ganoderma fornicatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Ganoderma fornicatum ?
-
 ?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Ganoderma fornicatum 0814 ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Ganoderma fornicatum ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Ganoderma fornicatum 0814 ?
-
 ?
guaiacol + O2
-
 Ganoderma fornicatum ?
-
 ?
guaiacol + O2
-
 Ganoderma fornicatum 0814 ?
-
 ?

Subunits

Subunits Comment Organism
? x + 63700, glycosylated recombinant enzyme, SDS-PAGE, x * 54100, about, sequence calculation Ganoderma fornicatum

Synonyms

Synonyms Comment Organism
LAC1
-
 Ganoderma fornicatum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Ganoderma fornicatum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 75 activity range Ganoderma fornicatum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 60 recombinant enzyme, pH 5.0 for 9 h, stable Ganoderma fornicatum
70
-
 recombinant enzyme, pH 5.0 for 9 h, loss of 80% activity Ganoderma fornicatum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5
-
 guaiacol recombinant enzyme, pH 3.0, 30°C Ganoderma fornicatum
38.5
-
 2,6-dimethoxyphenol recombinant enzyme, pH 3.0, 30°C Ganoderma fornicatum
65.6
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) recombinant enzyme, pH 3.0, 30°C Ganoderma fornicatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5
-
 with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) Ganoderma fornicatum
3
-
 with substrates 2,6-dimethoxyphenol and guaiacol Ganoderma fornicatum

pH Range

pH Minimum pH Maximum Comment Organism
2.5 5 activity range Ganoderma fornicatum

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.5 10 recombinant enzyme, rLac1 retains more than 80% of its activity in solutions within this range after a 24 h incubation at 25°C Ganoderma fornicatum

pI Value

Organism Comment pI Value Maximum pI Value
Ganoderma fornicatum sequence calculation
-
 5.3

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.9
-
 guaiacol recombinant enzyme, pH 3.0, 30°C Ganoderma fornicatum
140
-
 2,6-dimethoxyphenol recombinant enzyme, pH 3.0, 30°C Ganoderma fornicatum
630
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) recombinant enzyme, pH 3.0, 30°C Ganoderma fornicatum