Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 11.6% inhibition at 1 mM | Trametes pubescens | |
Ba2+ | 10% inhibition at 1 mM | Trametes pubescens | |
dithiothreitol | - |
Trametes pubescens | |
EDTA | - |
Trametes pubescens | |
L-cysteine | - |
Trametes pubescens | |
Sodium azide | - |
Trametes pubescens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Trametes pubescens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | activates slightly at 1 mM | Trametes pubescens | |
additional information | the laccase is highly metal-tolerant. No or poor effects by Mn2+, Na+, Zn2+, Mg2+, Ca2+, K+, Fe2+, and Fe3+ | Trametes pubescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
1 * 68000, SDS-PAGE | Trametes pubescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trametes pubescens | - |
collected from Chebaling Nature Reserve of Guangdong Province in China | - |
Trametes pubescens Cui 7571 | - |
collected from Chebaling Nature Reserve of Guangdong Province in China | - |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme 16fold from cell-free supernatant by ammonium sulfate fractionation, PEG 20000 concentration, anion exchange chromatography, and gel filtration | Trametes pubescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18.54 | - |
purified native extracellular enzyme, pH 5.0, 50°C | Trametes pubescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Trametes pubescens | ? | - |
? | |
2,6-dimethoxy phenol + O2 | - |
Trametes pubescens | ? | - |
? | |
4 catechol + O2 | - |
Trametes pubescens | 4 1,2-benzoquinone + 2 H2O | - |
? | |
4-hydroxy-3,5-dimethoxybenzaldehyde azine + O2 | i.e. syringaldazine | Trametes pubescens | ? | - |
? | |
ferulic acid + O2 | - |
Trametes pubescens | ? | - |
? | |
ferulic acid + O2 | - |
Trametes pubescens Cui 7571 | ? | - |
? | |
guaiacol + O2 | - |
Trametes pubescens | ? | - |
? | |
hydroquinone + O2 | - |
Trametes pubescens | ? | - |
? | |
hydroquinone + O2 | - |
Trametes pubescens Cui 7571 | ? | - |
? | |
L-DOPA + O2 | - |
Trametes pubescens | ? | - |
? | |
L-DOPA + O2 | - |
Trametes pubescens Cui 7571 | ? | - |
? | |
additional information | substrate specificity, overview. No activity with tyrosine. Dye decolorization capacity of an alkali-resistant and metal-tolerant laccase from Trametes pubescens, overview | Trametes pubescens | ? | - |
? | |
additional information | substrate specificity, overview. No activity with tyrosine. Dye decolorization capacity of an alkali-resistant and metal-tolerant laccase from Trametes pubescens, overview | Trametes pubescens Cui 7571 | ? | - |
? | |
phenol + O2 | - |
Trametes pubescens | ? | - |
? | |
pyrogallol + O2 | - |
Trametes pubescens | ? | - |
? | |
pyrogallol + O2 | - |
Trametes pubescens Cui 7571 | ? | - |
? | |
veratryl alcohol + O2 | - |
Trametes pubescens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 68000, SDS-PAGE | Trametes pubescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Trametes pubescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Trametes pubescens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 11 | activity range | Trametes pubescens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 10 | the purified enzyme is highly stable over a broad range of pH values ranging from pH 4.5 to pH 10.0, maintaining 75% of its original activity after incubation at 25°C for 72 h, it is highly stable and resistant under alkaline conditions, with pH values ranging from pH 7.0 to pH 10.0 | Trametes pubescens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8340 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 50°C | Trametes pubescens |