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Literature summary for 1.10.3.2 extracted from

  • Si, J.; Peng, F.; Cui, B.
    Purification, biochemical characterization and dye decolorization capacity of an alkali-resistant and metal-tolerant laccase from Trametes pubescens (2013), Biores. Technol., 128, 49-57.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 11.6% inhibition at 1 mM Trametes pubescens
Ba2+ 10% inhibition at 1 mM Trametes pubescens
dithiothreitol
-
Trametes pubescens
EDTA
-
Trametes pubescens
L-cysteine
-
Trametes pubescens
Sodium azide
-
Trametes pubescens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Trametes pubescens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ activates slightly at 1 mM Trametes pubescens
additional information the laccase is highly metal-tolerant. No or poor effects by Mn2+, Na+, Zn2+, Mg2+, Ca2+, K+, Fe2+, and Fe3+ Trametes pubescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
1 * 68000, SDS-PAGE Trametes pubescens

Organism

Organism UniProt Comment Textmining
Trametes pubescens
-
collected from Chebaling Nature Reserve of Guangdong Province in China
-
Trametes pubescens Cui 7571
-
collected from Chebaling Nature Reserve of Guangdong Province in China
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 16fold from cell-free supernatant by ammonium sulfate fractionation, PEG 20000 concentration, anion exchange chromatography, and gel filtration Trametes pubescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18.54
-
purified native extracellular enzyme, pH 5.0, 50°C Trametes pubescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Trametes pubescens ?
-
?
2,6-dimethoxy phenol + O2
-
Trametes pubescens ?
-
?
4 catechol + O2
-
Trametes pubescens 4 1,2-benzoquinone + 2 H2O
-
?
4-hydroxy-3,5-dimethoxybenzaldehyde azine + O2 i.e. syringaldazine Trametes pubescens ?
-
?
ferulic acid + O2
-
Trametes pubescens ?
-
?
ferulic acid + O2
-
Trametes pubescens Cui 7571 ?
-
?
guaiacol + O2
-
Trametes pubescens ?
-
?
hydroquinone + O2
-
Trametes pubescens ?
-
?
hydroquinone + O2
-
Trametes pubescens Cui 7571 ?
-
?
L-DOPA + O2
-
Trametes pubescens ?
-
?
L-DOPA + O2
-
Trametes pubescens Cui 7571 ?
-
?
additional information substrate specificity, overview. No activity with tyrosine. Dye decolorization capacity of an alkali-resistant and metal-tolerant laccase from Trametes pubescens, overview Trametes pubescens ?
-
?
additional information substrate specificity, overview. No activity with tyrosine. Dye decolorization capacity of an alkali-resistant and metal-tolerant laccase from Trametes pubescens, overview Trametes pubescens Cui 7571 ?
-
?
phenol + O2
-
Trametes pubescens ?
-
?
pyrogallol + O2
-
Trametes pubescens ?
-
?
pyrogallol + O2
-
Trametes pubescens Cui 7571 ?
-
?
veratryl alcohol + O2
-
Trametes pubescens ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 68000, SDS-PAGE Trametes pubescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Trametes pubescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Trametes pubescens

pH Range

pH Minimum pH Maximum Comment Organism
4.5 11 activity range Trametes pubescens

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 10 the purified enzyme is highly stable over a broad range of pH values ranging from pH 4.5 to pH 10.0, maintaining 75% of its original activity after incubation at 25°C for 72 h, it is highly stable and resistant under alkaline conditions, with pH values ranging from pH 7.0 to pH 10.0 Trametes pubescens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
8340
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 50°C Trametes pubescens