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Literature summary for 1.10.3.2 extracted from

  • Telke, A.A.; Ghodake, G.S.; Kalyani, D.C.; Dhanve, R.S.; Govindwar, S.P.
    Biochemical characteristics of a textile dye degrading extracellular laccase from a Bacillus sp. ADR (2011), Biores. Technol., 102, 1752-1756.
    View publication on PubMed
Search for more literature for 1.10.3.2

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol
-
 Bacillus sp. (in: Bacteria)
L-cysteine
-
 Bacillus sp. (in: Bacteria)
Sodium azide
-
 Bacillus sp. (in: Bacteria)

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Bacillus sp. (in: Bacteria)
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ purified laccase contains 2.7 mol/mol of copper Bacillus sp. (in: Bacteria)
additional information no activation or inhibition by 1 mM of MgCl2, CaCl2, MnCl2, ZnSO4, or CuSO4 Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
 x * 66000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) ADR
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native extracelluar enzyme 56fold by acetone precipitation and anion exchange chromatography Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.33
-
 purified native extracelluar enzyme, pH 4.0, 40°C Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,6-dimethoxyphenol + O2
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Bacillus sp. (in: Bacteria) ADR ?
-
 ?
guaiacol + O2
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
guaiacol + O2
-
 Bacillus sp. (in: Bacteria) ADR ?
-
 ?
hydroquinone + O2
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
hydroquinone + O2
-
 Bacillus sp. (in: Bacteria) ADR ?
-
 ?
L-DOPA + O2
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
L-DOPA + O2
-
 Bacillus sp. (in: Bacteria) ADR ?
-
 ?
additional information no activity with 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), syringaldazine, catechole, benzidine, 4-cresol, pyrogallol, caffeic acid and tyrosine. Decolorization of structurally different azo dyes using purified laccase: Methyl red, Reactive orange 16, Methyl orange, Sudan IV, Reactive red M5B, Direct brown MR, Reactive red 141, no decolorization of Congo red, Eriochrome black T, and Trypan blue Bacillus sp. (in: Bacteria) ?
-
 ?
additional information no activity with 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), syringaldazine, catechole, benzidine, 4-cresol, pyrogallol, caffeic acid and tyrosine. Decolorization of structurally different azo dyes using purified laccase: Methyl red, Reactive orange 16, Methyl orange, Sudan IV, Reactive red M5B, Direct brown MR, Reactive red 141, no decolorization of Congo red, Eriochrome black T, and Trypan blue Bacillus sp. (in: Bacteria) ADR ?
-
 ?
o-tolidine + O2 best substrate Bacillus sp. (in: Bacteria) ?
-
 ?

Subunits

Subunits Comment Organism
? x * 66000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable up to Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
 with substrate o-tolidine Bacillus sp. (in: Bacteria)
4
-
 with substrate 2,6-dimethoxyphenol Bacillus sp. (in: Bacteria)
5
-
 with substrate guaiacol Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9 stable at Bacillus sp. (in: Bacteria)