Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as soluble intein fusion protein | Shigella dysenteriae |
Protein Variants | Comment | Organism |
---|---|---|
E106F | site-directed mutagenesis with additional deletion of residues L351-G378, the mutant WlacD shows 3.5fold increased activity and enhanced thermal stability compared to the wild-type enzyme | Shigella dysenteriae |
E106F | site-directed mutagenesis, the mutant WlacS shows 2.2fold increased activity compared to the wild-type enzyme | Shigella dysenteriae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | completely inhibited by 200 mM | Shigella dysenteriae | |
NaN3 | completely inhibited by 50 mM | Shigella dysenteriae | |
Pb2+ | inhibits 50% at 10 mM | Shigella dysenteriae | |
SDS | completely inhibited by 32 mM | Shigella dysenteriae | |
thiolglycolic acid | completely inhibited by 60 mM | Shigella dysenteriae | |
Zn2+ | inhibits 40% at 10 mM | Shigella dysenteriae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.086 | - |
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) | pH 4.0, 25°C, recombinant wild-type enzyme | Shigella dysenteriae | |
0.112 | - |
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) | pH 4.0, 25°C, recombinant mutant WlacS | Shigella dysenteriae | |
0.129 | - |
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) | pH 4.0, 25°C, recombinant mutant WlacD | Shigella dysenteriae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | stimulates the wild-type enzyme by 6% at 10 mM, 14.5% at 100 mM, required for activity | Shigella dysenteriae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
107000 | - |
recombinant mutant WlacD | Shigella dysenteriae |
110000 | - |
recombinant wild-type enzyme and mutant WlacS | Shigella dysenteriae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Shigella dysenteriae | - |
strain W202, a clinically isolated wild-type strain | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
24.4 | - |
purified recombinant wild-type Wlac in presence of 100 mM Cu2+ | Shigella dysenteriae |
52.9 | - |
purified recombinant mutantWlacS in presence of 100 mM Cu2+ | Shigella dysenteriae |
85.1 | - |
purified recombinant mutantWlacD in presence of 100 mM Cu2+ | Shigella dysenteriae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Shigella dysenteriae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Wlac | - |
Shigella dysenteriae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Shigella dysenteriae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
25 | purified recombinant enzyme, stable | Shigella dysenteriae |
35 | - |
purified recombinant enzyme, 2 h, loss of 30% activity | Shigella dysenteriae |
45 | - |
purified recombinant enzyme, 1 h, loss of 30% activity | Shigella dysenteriae |
75 | - |
purified recombinant enzyme, 5 min, loss of 90% activity | Shigella dysenteriae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18.2 | - |
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) | pH 4.0, 25°C, recombinant wild-type enzyme | Shigella dysenteriae | |
36.5 | - |
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) | pH 4.0, 25°C, recombinant mutant WlacS | Shigella dysenteriae | |
50.9 | - |
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) | pH 4.0, 25°C, recombinant mutant WlacD | Shigella dysenteriae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2.5 | - |
- |
Shigella dysenteriae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
0.5 | 8 | activity range | Shigella dysenteriae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | - |
4-25°C, 24 h, loss of 40% activity | Shigella dysenteriae |
7 | - |
4-25°C, 24 h, stable | Shigella dysenteriae |
8 | - |
4°C, 24 h, loss of 25% activity, at 25°C oss of 55% activity | Shigella dysenteriae |