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Literature summary for 1.10.3.2 extracted from

  • Junghanns, C.; Pecyna, M.J.; Boehm, D.; Jehmlich, N.; Martin, C.; von Bergen, M.; Schauer, F.; Hofrichter, M.; Schlosser, D.
    Biochemical and molecular genetic characterisation of a novel laccase produced by the aquatic ascomycete Phoma sp. UHH 5-1-03 (2009), Appl. Microbiol. Biotechnol., 84, 1095-1105.
    View publication on PubMed
Search for more literature for 1.10.3.2

Inhibitors

Inhibitors Comment Organism Structure
acetic acid
-
 Phoma sp.
acetone 31% (v/v) 50% inhibition Phoma sp.
acetonitrile 4.5% (v/v) 50% inhibition Phoma sp.
DMSO 40% (v/v) 50% inhibition Phoma sp.
EDTA
-
 Phoma sp.
ethanol 52% (v/v) 50% inhibition Phoma sp.
formic acid
-
 Phoma sp.
Isopropanol 60% (v/v) 50% inhibition Phoma sp.
methanol 52% (v/v) 50% inhibition Phoma sp.
Na2CO3
-
 Phoma sp.
NaCl
-
 Phoma sp.
NaF
-
 Phoma sp.
NaN3
-
 Phoma sp.
Urea
-
 Phoma sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Phoma sp.
0.008
-
 2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) pH 2.5, 65°C Phoma sp.
0.02
-
 syringaldazine pH 5.0, 65°C Phoma sp.
0.266
-
 2,6-dimethoxyphenol pH 4.0, 65°C Phoma sp.
0.284
-
 anthraquinonic dye Acid Blue 62 pH 5.5, 65°C Phoma sp.
0.648
-
 guaiacol pH 5.5, 65°C Phoma sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Phoma sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
copper a blue copper-containing oxidoreductase Phoma sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000 151200 about, gel filtration and mass spectrometry Phoma sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Phoma sp. laccases are able to oxidize a variety of substrates, ranging from mono- and polyphenols over aminophenols, methoxyphenols and aromatic amines to polycyclic aromatic hydrocarbons and certain lignin moieties ?
-
 ?
additional information Phoma sp. UHH 5-1-03 / DSM 22425 laccases are able to oxidize a variety of substrates, ranging from mono- and polyphenols over aminophenols, methoxyphenols and aromatic amines to polycyclic aromatic hydrocarbons and certain lignin moieties ?
-
 ?

Organism

Organism UniProt Comment Textmining
Phoma sp. B3TLA6 isolated from Saale river Germany
-
Phoma sp. UHH 5-1-03 / DSM 22425 B3TLA6 isolated from Saale river Germany
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein N-glycosylation site prediction reveals five putative sites, and nine putative sites for O-glycosylation, overview Phoma sp.

Purification (Commentary)

Purification (Comment) Organism
native enzyme 117fold by hydrophobic interaction chromatography and gel filtration Phoma sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.1
-
 purified enzyme Phoma sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Phoma sp. ?
-
 ?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Phoma sp. UHH 5-1-03 / DSM 22425 ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Phoma sp. ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Phoma sp. UHH 5-1-03 / DSM 22425 ?
-
 ?
anthraquinonic dye Acid Blue 62 + O2
-
 Phoma sp. ?
-
 ?
guaiacol + O2
-
 Phoma sp. ?
-
 ?
guaiacol + O2
-
 Phoma sp. UHH 5-1-03 / DSM 22425 ?
-
 ?
additional information laccases are able to oxidize a variety of substrates, ranging from mono- and polyphenols over aminophenols, methoxyphenols and aromatic amines to polycyclic aromatic hydrocarbons and certain lignin moieties Phoma sp. ?
-
 ?
additional information laccases are able to oxidize a variety of substrates, ranging from mono- and polyphenols over aminophenols, methoxyphenols and aromatic amines to polycyclic aromatic hydrocarbons and certain lignin moieties Phoma sp. UHH 5-1-03 / DSM 22425 ?
-
 ?
syringaldazine + O2
-
 Phoma sp. ?
-
 ?
syringaldazine + O2
-
 Phoma sp. UHH 5-1-03 / DSM 22425 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 74000-75600, about, mass spectrometry and SDS-PAGE, laccase undergoes pH-dependent dimerisation Phoma sp.
More the enzyme contains a signal sequence and a C-terminal conserved DSGL motif, tryptic peptide analysis, overview Phoma sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Phoma sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 45 the enzyme is most stable between 4°C and room temperature. After 288 h, the recovered activities correspond to approximately 51% at 4°C, 57% at 15°C and 49% at room temperature of the initial activity. The laccase is nearly completely inactivated at 35°C after 288 h and at 45°C after 24 h Phoma sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5
-
 syringaldazine pH 5.0, 65°C Phoma sp.
4.3
-
 2,6-dimethoxyphenol pH 4.0, 65°C Phoma sp.
4.6
-
 guaiacol pH 5.5, 65°C Phoma sp.
10.6
-
 2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) pH 2.5, 65°C Phoma sp.
32.2
-
 anthraquinonic dye Acid Blue 62 pH 5.5, 65°C Phoma sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5 5.5 the optimal pH values for the different substrates 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid, 2,6-dimethoxyphenol, guaiacol, syringaldazine, and anthraquinonic dye Acid Blue 62 oxidation are pH 2.5, pH 4.5, pH 5.0, pH 5.5 and pH 5.5, respectively Phoma sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 8 activity range, at pH 7.0, about 17%, 24%, 33%, 23% and 21% of the maximum oxidation rate is observed for substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid, 2,6-dimethoxyphenol, guaiacol, syringaldazine, and anthraquinonic dye Acid Blue 62, respectively, while approximately 2%, 8%, 15%, 4% and 17% of the maximum activity in oxidation of these substrates is obtained at pH 8.0 Phoma sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8 the laccase is most stable at pH 4.0, pH 7.0, and 8.0, retaining about 57%, 62%, and 65% of the initial activity, respectively, after 355 h. The enzyme is less stable at pH 5.0 and pH 6.0 and is completely inactivated within 355 h at pH 2.0 Phoma sp.

pI Value

Organism Comment pI Value Maximum pI Value
Phoma sp. isoelectric focussing
-
 8.3

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.11
-
 pH 4.0, 65°C Phoma sp. NaN3
0.23
-
 pH 4.0, 65°C Phoma sp. formic acid
3.7
-
 pH 4.0, 65°C Phoma sp. NaF
19
-
 pH 4.0, 65°C Phoma sp. EDTA
69
-
 pH 4.0, 65°C Phoma sp. Na2CO3
80
-
 pH 4.0, 65°C Phoma sp. NaCl
2250
-
 pH 4.0, 65°C Phoma sp. Urea