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Literature summary for 1.10.3.2 extracted from

  • Tlecuitl-Beristain, S.; Sanchez, C.; Loera, O.; Robson, G.D.; Diaz-Godinez, G.
    Laccases of Pleurotus ostreatus observed at different phases of its growth in submerged fermentation: production of a novel laccase isoform (2008), Mycol. Res., 112, 1080-1084.
    View publication on PubMed

Application

Application Comment Organism
environmental protection LI1 shows activity over a broad range of pH and temperature, which may make it useful in the biodegradation of phenolic compounds present in wastewater from several industrial processes Pleurotus ostreatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
2,6-dimethoxyphenol
-
Pleurotus ostreatus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Pleurotus ostreatus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43700
-
x * 43700, SDS-PAGE Pleurotus ostreatus

Organism

Organism UniProt Comment Textmining
Pleurotus ostreatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pleurotus ostreatus

Source Tissue

Source Tissue Comment Organism Textmining
culture medium laccase activity is positively correlated to the growth of the fungus. Four laccase isoforms are secreted by the fungus, and tentatively named LI1, LI2, LI3 and LI4. LI2, LI3 and LI4 are produced during the stationary phase (between 408 and 456 h approximately) while LI1 is produced during the lag, exponential and stationary phases of growth. Maximal laccase activity is observed at the beginning of the stationary phase Pleurotus ostreatus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,6-dimethoxyphenol + O2
-
Pleurotus ostreatus ?
-
?

Subunits

Subunits Comment Organism
? x * 43700, SDS-PAGE Pleurotus ostreatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 55 isoenzyme LI1 Pleurotus ostreatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
isoenzyme LI1 Pleurotus ostreatus

pH Range

pH Minimum pH Maximum Comment Organism
2 8 60°C, more than 50% of maximal activity in the pH-range 2-8 Pleurotus ostreatus

pI Value

Organism Comment pI Value Maximum pI Value
Pleurotus ostreatus isoenzyme LI1
-
2.3