Application | Comment | Organism |
---|---|---|
environmental protection | the stability of this laccase against metal ions makes the enzyme an efficient agent in the treatment of wastewater containing heavy metals | Perenniporia tephropora |
General Stability | Organism |
---|---|
laccase is stable in the presence of some metal ions such as Cu2+, Co2+, Ca2+, Cd2+, Mg2+, Mn2+, Mo2+, Ni2+, Li+ and Al3+ | Perenniporia tephropora |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 100 mM, 92% inhibition | Perenniporia tephropora | |
Ca2+ | 100 mM, 43% inhibition | Perenniporia tephropora | |
Cd2+ | 100 mM, 92% inhibition | Perenniporia tephropora | |
Co2+ | 100 mM, 51% inhibition | Perenniporia tephropora | |
EDTA | 0.1 mM, complete inhibition | Perenniporia tephropora | |
L-cysteine | 5 mM, complete inhibition | Perenniporia tephropora | |
Mg2+ | 100 mM, 33% inhibition | Perenniporia tephropora | |
Mn2+ | 100 mM, 44% inhibition | Perenniporia tephropora | |
Mo2+ | 100 mM, 82% inhibition | Perenniporia tephropora | |
additional information | no inhibition: SDS, NaBr | Perenniporia tephropora | |
NaN3 | 0.1 mM, complete inhibition | Perenniporia tephropora | |
Ni2+ | 100 mM, 52% inhibition | Perenniporia tephropora | |
p-coumarate | 0.1 mM, complete inhibition | Perenniporia tephropora |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
2,2'-azino-di(3-ethyl-benzthiazoline-6-sulfonate) | - |
Perenniporia tephropora | |
7.3 | - |
2,6-dimethoxyphenol | - |
Perenniporia tephropora |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Perenniporia tephropora | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | activates | Perenniporia tephropora | |
Li+ | activates | Perenniporia tephropora |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
1 * 63000, SDS-PAGE | Perenniporia tephropora |
65000 | - |
gel filtration | Perenniporia tephropora |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Perenniporia tephropora | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Perenniporia tephropora |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition | malt extract is the carbon source which yields the highest laccase production, with a maximum of laccase production reached after 9 days of cultivation. The nitrogen source soya peptone yields the highest laccase production | Perenniporia tephropora | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
181 | - |
- |
Perenniporia tephropora |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Perenniporia tephropora | ? + H2O | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Perenniporia tephropora | ? + H2O | - |
? | |
3,4-dihydroxyphenylacetate + O2 | - |
Perenniporia tephropora | ? + H2O | - |
? | |
caffeate + O2 | - |
Perenniporia tephropora | ? + H2O | - |
? | |
catechol + O2 | - |
Perenniporia tephropora | ? + H2O | - |
? | |
ferulate + O2 | - |
Perenniporia tephropora | ? + H2O | - |
? | |
gallate + O2 | - |
Perenniporia tephropora | ? + H2O | - |
? | |
guaiacol + O2 | - |
Perenniporia tephropora | ? + H2O | - |
? | |
additional information | the crude enzyme as well as the purified laccase is able to decolourize dyes from the textile industries, including remazol brilliant blue R, neolane blue and neolane pink | Perenniporia tephropora | ? | - |
? | |
protocatechuate + O2 | - |
Perenniporia tephropora | ? + H2O | - |
? | |
pyrogallol + O2 | - |
Perenniporia tephropora | ? + H2O | - |
? | |
syringate + O2 | - |
Perenniporia tephropora | ? + H2O | - |
? | |
vanillate + O2 | - |
Perenniporia tephropora | ? + H2O | - |
? | |
vanillin + O2 | - |
Perenniporia tephropora | ? + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 63000, SDS-PAGE | Perenniporia tephropora |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
inactivated rapidly above | Perenniporia tephropora |
70 | - |
2 h, complete inactivation | Perenniporia tephropora |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
substrate: 2,6-dimethoxyphenol | Perenniporia tephropora |
5 | - |
substrate: 2,2'-azino-di(3-ethyl-benzthiazoline-6-sulfonate) | Perenniporia tephropora |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | - |
more stable at alkaline values of pH (8.0) than at acidic values | Perenniporia tephropora |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Perenniporia tephropora | isoelectric focusing | - |
3.3 |