Any feedback?
Literature summary for 1.10.3.2 extracted from
- Biochemical characterization of the coexisting tyrosinase and laccase in the soil bacterium Pseudomonas putida F6 (2007), Enzyme Microb. Technol., 40, 1435-1441.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
syringaldazine | enzyme has a higher affinity for syringaldazine than for L-dopa | Pseudomonas putida |  |
| 0.84 | - |
L-Dopa | enzyme has a higher affinity for syringaldazine than for L-dopa | Pseudomonas putida | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Pseudomonas putida | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| copper | - |
Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59000 | - |
indicated by gel filtration chromatography | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
soil bacterium. The coexistence of this tyrosinase with a laccase in Pseudomonas putida F6 is reported and both activities characterised | - |
| Pseudomonas putida F6 | - |
soil bacterium. The coexistence of this tyrosinase with a laccase in Pseudomonas putida F6 is reported and both activities characterised | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| combination of anion exchange chromatography, gel filtration and gel slicing | Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.011 | - |
CE, purification of Pseudomonas putida F6 laccase-type activity | Pseudomonas putida |
| 0.061 | - |
DEAE, purification of Pseudomonas putida F6 laccase-type activity | Pseudomonas putida |
| 0.573 | - |
Resource Q, purification of Pseudomonas putida F6 laccase-type activity | Pseudomonas putida |
| 4.57 | - |
gel filtration, purification of Pseudomonas putida F6 laccase-type activity | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-DOPA + O2 | - |
Pseudomonas putida | ? + H2O | - |
? | |
| L-DOPA + O2 | - |
Pseudomonas putida F6 | ? + H2O | - |
? | |
| syringaldazine + O2 | - |
Pseudomonas putida | ? | - |
? | |
| syringaldazine + O2 | - |
Pseudomonas putida F6 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| laccase | - |
Pseudomonas putida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Pseudomonas putida |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
stable over a broad range of temperatures and are most stable at 30°C | Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at, most stable at pH 7.0 | Pseudomonas putida |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
