Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis | Cyathus bulleri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | competitive, complete inhibition | Cyathus bulleri | |
cetyltrimethyl ammonium bromide | 50% inhibition | Cyathus bulleri | |
HgCl2 | competitive, complete inhibition | Cyathus bulleri | |
KCN | competitive, complete inhibition | Cyathus bulleri | |
NaN3 | competitive, complete inhibition | Cyathus bulleri | |
SDS | competitive, complete inhibition | Cyathus bulleri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14 | - |
ferulic acid | pH 5.2, 45°C | Cyathus bulleri | |
22 | - |
guaiacol | pH 5.2, 45°C | Cyathus bulleri | |
48.6 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 5.2, 45°C | Cyathus bulleri | |
56 | - |
2,6-dimethoxyphenol | pH 5.2, 45°C | Cyathus bulleri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | the gene sequence encodes copper-binding domains I and II | Cyathus bulleri | |
additional information | no effect by EDTA | Cyathus bulleri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
gel filtration | Cyathus bulleri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cyathus bulleri | Q8X1D1 | birds nest fungus | - |
Purification (Comment) | Organism |
---|---|
native enzyme 95.7fold to homogeneity by anion exchange chromatography and gel filtration | Cyathus bulleri |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Cyathus bulleri | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
226 | - |
purified enzyme | Cyathus bulleri |
Storage Stability | Organism |
---|---|
4°C, purified enzyme, 50 mM citrate phosphate, pH 5.0, no loss in activity after 1 month | Cyathus bulleri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Cyathus bulleri | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Cyathus bulleri | 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O | - |
? | |
3,4-dihydroxybenzoic acid + O2 | - |
Cyathus bulleri | ? | - |
? | |
ferulic acid + O2 | - |
Cyathus bulleri | ? | - |
? | |
guaiacol + O2 | - |
Cyathus bulleri | ? | - |
? | |
additional information | substrate specificity, overview, no activity with tyrosine, phenol, 2,4-dichlorophenol, and 5-sulphosalycylic acid | Cyathus bulleri | ? | - |
? | |
syringaldehyde + O2 | - |
Cyathus bulleri | ? | - |
? | |
vanillin + O2 | - |
Cyathus bulleri | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60000, SDS-PAGE | Cyathus bulleri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Cyathus bulleri |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 70 | stable at, inactivation above | Cyathus bulleri |
80 | - |
complete inactivation | Cyathus bulleri |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.2 | - |
ferulic acid | pH 5.2, 45°C | Cyathus bulleri | |
5.5 | - |
guaiacol | pH 5.2, 45°C | Cyathus bulleri | |
95.6 | - |
guaiacol | pH 5.2, 45°C | Cyathus bulleri | |
180 | - |
2,6-dimethoxyphenol | pH 5.2, 45°C | Cyathus bulleri | |
204 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 5.2, 45°C | Cyathus bulleri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.2 | - |
- |
Cyathus bulleri |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 6 | sharp decline in activity above pH 6.0 | Cyathus bulleri |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cyathus bulleri | isoelectric focusing | - |
3.7 |