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Literature summary for 1.10.3.2 extracted from

  • Zhang, M.; Wu, F.; Wei, Z.; Xiao, Y.; Gong, W.
    Characterization and decolorization ability of a laccase from Panus rudis (2006), Enzyme Microb. Technol., 39, 92-97.
No PubMed abstract available
Search for more literature for 1.10.3.2

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparison Panus rudis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 4.5, 37°C Panus rudis

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ His64 and His399 coordinate with T2 Cu and the other six histidines at positions 66, 109, 111, 401, 451 and 453 coordinate with the T3 Cu pair, a hydrogen bond between Glu460 and Ser113 causes a longer Cu1-N-His458 bond at T1 site, Cu2+ binding structure, overview Panus rudis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 x * 58000, glycosylated enzyme, SDS-PAGE, x * 53000, deglycosylated enzyme, SDS-PAGE Panus rudis
58000
-
 x * 58000, glycosylated enzyme, SDS-PAGE, x * 53000, deglycosylated enzyme, SDS-PAGE Panus rudis

Organism

Organism UniProt Comment Textmining
Panus rudis Q6T2T5
-
-
Panus rudis 5.34 Q6T2T5
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein contains 8% carbohydrate, five potential N-glycosylation sites Asn-X-Thr/Ser at positions 217, 387, 435, 471 and 477 Panus rudis

Purification (Commentary)

Purification (Comment) Organism
native enzyme to homogeneity Panus rudis

Source Tissue

Source Tissue Comment Organism Textmining
additional information constitutive production in defined shaken liquid culture without induction Panus rudis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
460
-
 purified enzyme, substrate 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Panus rudis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Panus rudis ?
-
 ?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Panus rudis 5.34 ?
-
 ?
additional information Acid Green 27, Acid Violet 7 and Indigo Carmine are used in a dye decolorization assay, overview Panus rudis ?
-
 ?
additional information Acid Green 27, Acid Violet 7 and Indigo Carmine are used in a dye decolorization assay, overview Panus rudis 5.34 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 58000, glycosylated enzyme, SDS-PAGE, x * 53000, deglycosylated enzyme, SDS-PAGE Panus rudis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Panus rudis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
-
 Panus rudis

pI Value

Organism Comment pI Value Maximum pI Value
Panus rudis
-
-
 3.5