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Literature summary for 1.10.3.2 extracted from

  • Rosconi, F.; Fraguas, L.F.; Martinez-Drets, G.; Castro-Sowinski, S.
    Purification and characterization of a periplasmic laccase produced by Sinorhizobium meliloti (2005), Enzyme Microb. Technol., 36, 800-807.
No PubMed abstract available

General Stability

General Stability Organism
ammonium sulfate stabilizes Sinorhizobium meliloti
no satnilization by EDTA and Cu2+ Sinorhizobium meliloti

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
Sinorhizobium meliloti
Cu2+
-
Sinorhizobium meliloti
Fe3+
-
Sinorhizobium meliloti
Mn2+
-
Sinorhizobium meliloti
NaCl
-
Sinorhizobium meliloti
NaCN
-
Sinorhizobium meliloti
NaF
-
Sinorhizobium meliloti
reduced glutathione
-
Sinorhizobium meliloti

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Sinorhizobium meliloti
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
(NH4)2SO4 4fold activation Sinorhizobium meliloti
imidazole about 2fold activation Sinorhizobium meliloti
K2SO4 3.2fold activation Sinorhizobium meliloti
Na2SO4 3.5fold activation Sinorhizobium meliloti

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
2 * 45000, SDS-PAGE Sinorhizobium meliloti

Organism

Organism UniProt Comment Textmining
Sinorhizobium meliloti
-
-
-
Sinorhizobium meliloti CE52G
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 29fold by a two-step procedure involving heat treatment and immobilized metal affinity chromatography Sinorhizobium meliloti

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.402
-
purified enzyme Sinorhizobium meliloti

Storage Stability

Storage Stability Organism
the purified enzyme retains its original activity after 24 h of incubation at pH between 4.0 and 8.0 and after 3 h of incubation at 70°C, pH 7.2 and supplemented with 1.3 M (NH4)2SO4 Sinorhizobium meliloti

Subunits

Subunits Comment Organism
dimer 2 * 45000, SDS-PAGE Sinorhizobium meliloti

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
the purified enzyme retains its original activity after 24 h of incubation at pH between 4.0 and 8.0 and after 3 h of incubation at 70°C, pH 7.2 and supplemented with 1.3 M (NH4)2SO4 Sinorhizobium meliloti
75
-
purified enzyme, inactivation Sinorhizobium meliloti

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8 the purified enzyme retains its original activity after 24 h of incubation at pH between 4.0 and 8.0 and after 3 h of incubation at 70°C, pH 7.2 and supplemented with 1.3 M (NH4)2SO4 Sinorhizobium meliloti

pI Value

Organism Comment pI Value Maximum pI Value
Sinorhizobium meliloti isoelectric focusing
-
6.2