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Literature summary for 1.10.3.2 extracted from

  • Schroeder, M.; Heumann, S.; Silva, C.J.; Cavaco-Paulo, A.; Guebitz, G.M.
    Specificities of a chemically modified laccase from Trametes hirsuta on soluble and cellulose-bound substrates (2006), Biotechnol. Lett., 28, 741-747.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information enzyme adsorption to cellulose fibers from cotton and denim, attachment of PEG of 1.1-5.0 kDa to the purified enzyme, overview Trametes hirsuta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00134
-
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 4.5, 50°C, free enzyme Trametes hirsuta
0.005
-
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 4.5, 50°C, enzyme conjugated with 1.1 kDa PEG Trametes hirsuta
0.00632
-
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 4.5, 50°C, enzyme conjugated with 2 kDa PEG Trametes hirsuta
0.00704
-
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 4.5, 50°C, enzyme conjugated with 5 kDa PEG Trametes hirsuta

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
free enzyme, gel filtration Trametes hirsuta
85000
-
enzyme conjugated with 1.1 kDa PEG, gel filtration Trametes hirsuta
177000
-
enzyme conjugated with 2 kDa PEG, gel filtration Trametes hirsuta
269000
-
enzyme conjugated with 5 kDa PEG, gel filtration Trametes hirsuta

Organism

Organism UniProt Comment Textmining
Trametes hirsuta
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Trametes hirsuta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
Trametes hirsuta ?
-
?
additional information substrate specificities of a laccase chemically modified by attachment of PEG on soluble and cellulose-bound dye substrates, overview Trametes hirsuta ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 52000, SDS-PAGE Trametes hirsuta

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Trametes hirsuta

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
purified free enzyme half-life: 140 min, purified enzyme conjugated with 1.1 kDa PEG half-life: 70 min, purified enzyme conjugated with 2 kDa PEG half-life: 150 min, purified enzyme conjugated with 5.0 kDa PEG half-life: 210 min Trametes hirsuta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Trametes hirsuta

pH Range

pH Minimum pH Maximum Comment Organism
3 6.5
-
Trametes hirsuta

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 6.5 stable, purified enzyme Trametes hirsuta