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Literature summary for 1.10.3.2 extracted from
- Molecular and biochemical characterization of a highly stable bacterial laccase that occurs as a structural component of the Bacillus subtilis endospore coat (2002), J. Biol. Chem., 277, 18849-18859.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H497A | copper center, no significant changes | Bacillus subtilis |
| M502L | copper center, no significant changes | Bacillus subtilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
syringaldazine | - |
Bacillus subtilis |  |
| 0.11 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | - |
Bacillus subtilis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
1 * 55000, SDS-PAGE, isoenzymes I and II | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Bacillus subtilis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Bacillus subtilis | ? | - |
? | |
| syringaldazine + O2 | - |
Bacillus subtilis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 55000, SDS-PAGE, isoenzymes I and II | Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
half life 2-4 h | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
or less, substrate 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | Bacillus subtilis |
| 7 | - |
substrate syringaldazine | Bacillus subtilis |
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