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Literature summary for 1.10.3.11 extracted from
- Mutagenesis of the Sauromatum guttatum alternative oxidase reveals features important for oxygen binding and catalysis (2010), Biochim. Biophys. Acta, 1797, 732-737.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant enzymes in Schizosaccharomyces pombe strain Sp.011, subcloning in Escherichia coli strains JM101 and 110 | Sauromatum venosum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C172A | site-directed mutagenesis, the mutant shows reduced activity and oxygen affinity compared to the wild-type enzyme | Sauromatum venosum |
| T179A | site-directed mutagenesis, the mutant shows reduced activity and oxygen affinity compared to the wild-type enzyme | Sauromatum venosum |
| W206F | site-directed mutagenesis, inactive mutant | Sauromatum venosum |
| W206Y | site-directed mutagenesis, inactive mutant | Sauromatum venosum |
| Y253F | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Sauromatum venosum |
| Y299F | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Sauromatum venosum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| octyl gallate | - |
Sauromatum venosum |  |
| sialic acid | - |
Sauromatum venosum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0046 | - |
O2 | mutant T179A, pH and temperature not specified in the publication | Sauromatum venosum | |
| 0.0092 | - |
O2 | mutant C172A, pH and temperature not specified in the publication | Sauromatum venosum | |
| 0.017 | - |
O2 | mutant Y299F, pH and temperature not specified in the publication | Sauromatum venosum | |
| 0.018 | - |
O2 | wild-type enzyme, pH and temperature not specified in the publication | Sauromatum venosum | |
| 0.0205 | - |
O2 | mutant Y253F, pH and temperature not specified in the publication | Sauromatum venosum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Sauromatum venosum | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ubiquinol + O2 | Sauromatum venosum | - |
2 ubiquinone + 2 H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sauromatum venosum | P22185 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ubiquinol + O2 | - |
Sauromatum venosum | 2 ubiquinone + 2 H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alternative oxidase | - |
Sauromatum venosum |
| AOX | - |
Sauromatum venosum |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00007 | - |
mutant Y299F, pH and temperature not specified in the publication | Sauromatum venosum | octyl gallate | |
| 0.00008 | - |
mutants C172A and T179A, pH and temperature not specified in the publication | Sauromatum venosum | octyl gallate | |
| 0.00009 | - |
wild-type enzyme, pH and temperature not specified in the publication | Sauromatum venosum | octyl gallate | |
| 0.0001 | - |
mutant Y253F, pH and temperature not specified in the publication | Sauromatum venosum | octyl gallate | |
| 0.012 | - |
mutant T179A, pH and temperature not specified in the publication | Sauromatum venosum | sialic acid | |
| 0.016 | - |
wild-type enzyme, pH and temperature not specified in the publication | Sauromatum venosum | sialic acid | |
| 0.018 | - |
mutant Y299F, pH and temperature not specified in the publication | Sauromatum venosum | sialic acid | |
| 0.019 | - |
mutant C172A, pH and temperature not specified in the publication | Sauromatum venosum | sialic acid | |
| 0.02 | - |
mutant Y253F, pH and temperature not specified in the publication | Sauromatum venosum | sialic acid | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme is found in mitochondria of all higher plants studied to date | Sauromatum venosum |
| physiological function | the alternative oxidase (AOX) is a non-protonmotive ubiquinol oxidase | Sauromatum venosum |
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