Literature summary for 1.10.3.11 extracted from

Mogi, T.; Ano, Y.; Nakatsuka, T.; Toyama, H.; Muroi, A.; Miyoshi, H.; Migita, C.T.; Ui, H.; Shiomi, K.; Omura, S.; Kita, K.; Matsushita, K.
Biochemical and spectroscopic properties of cyanide-insensitive quinol oxidase from Gluconobacter oxydans (2009), J. Biochem., 146, 263-271.

Search for more literature for 1.10.3.11

Inhibitors

Inhibitors	Comment	Organism	Structure
2-Heptyl-4-hydroxyquinoline-N-oxide	-	Gluconobacter oxydans
antimycin A	56% residual activity at 0.005 mg/ml; 56% residual activity at 0.005 mg/ml	Gluconobacter oxydans
aurachin C 1-10	15% residual activity at 0.005 mg/ml; 15% residual activity at 0.005 mg/ml	Gluconobacter oxydans
aurachin D	16% residual activity at 0.005 mg/ml; 16% residual activity at 0.005 mg/ml	Gluconobacter oxydans
cyanide	quinol oxidase activity of the membranes from the wild type shows a biphasic dependence on the cyanide concentration; quinol oxidase activity of the membranes from the wild type shows a biphasic dependence on the cyanide concentration	Gluconobacter oxydans
Gramicidin S	67% residual activity at 0.005 mg/ml; 67% residual activity at 0.005 mg/ml	Gluconobacter oxydans
LL-Z1272gamma	69% residual activity at 0.005 mg/ml; 69% residual activity at 0.005 mg/ml	Gluconobacter oxydans
additional information	not inhibited by ascofuranone; not inhibited by ascofuranone	Gluconobacter oxydans
Piericidin A	14% residual activity at 0.005 mg/ml; 14% residual activity at 0.005 mg/ml	Gluconobacter oxydans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0403	-	ubiquinol-1	at 25°C in 50 mM potassium phosphate (pH 6.5)	Gluconobacter oxydans

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Gluconobacter oxydans	16020	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
37813	-	1 * 53583 + 1 * 37813, calculated from amino acid sequence	Gluconobacter oxydans
44000	-	1 * 54000 + 1 * 44000, SDS-PAGE	Gluconobacter oxydans
53583	-	1 * 53583 + 1 * 37813, calculated from amino acid sequence	Gluconobacter oxydans
54000	-	1 * 54000 + 1 * 44000, SDS-PAGE	Gluconobacter oxydans

Organism

Organism	UniProt	Comment	Textmining
Gluconobacter oxydans	-	subunit I; formerly Gluconobacter suboxydans strain IFO12528	-
Gluconobacter oxydans	Q5FU84	subunit II; formerly Gluconobacter suboxydans strain IFO12528. The enzyme from strain 621H is expressed in strain NBRC3172	-
Gluconobacter oxydans 621H	Q5FU84	subunit II; formerly Gluconobacter suboxydans strain IFO12528. The enzyme from strain 621H is expressed in strain NBRC3172	-
Gluconobacter oxydans NBRC 3172	-	subunit I; formerly Gluconobacter suboxydans strain IFO12528	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ubiquinol-1 + O2	-	Gluconobacter oxydans	ubiquinone-1 + H2O	-	?
ubiquinol-1 + O2	-	Gluconobacter oxydans 621H	ubiquinone-1 + H2O	-	?
ubiquinol-1 + O2	-	Gluconobacter oxydans NBRC 3172	ubiquinone-1 + H2O	-	?

Subunits

Subunits	Comment	Organism
heterodimer	1 * 53583 + 1 * 37813, calculated from amino acid sequence	Gluconobacter oxydans
heterodimer	1 * 54000 + 1 * 44000, SDS-PAGE	Gluconobacter oxydans

Synonyms

Synonyms	Comment	Organism
CIO	-	Gluconobacter oxydans
CioAB	-	Gluconobacter oxydans
cyanide-insensitive oxidase	-	Gluconobacter oxydans
cyanide-insensitive quinol oxidase	-	Gluconobacter oxydans

Cofactor

Cofactor	Comment	Organism	Structure
heme	heme b558, heme b595 and heme d	Gluconobacter oxydans

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.00004	-	at 25°C in 50 mM potassium phosphate (pH 6.5)	Gluconobacter oxydans	aurachin C 1-10
0.0007	-	at 25°C in 50 mM potassium phosphate (pH 6.5)	Gluconobacter oxydans	Piericidin A
0.008	-	quinol oxidase activity of the membranes from the wild type shows a biphasic dependence on the cyanide concentration with the IC50 of 0.008 mM (relative amplitude, 21%) and 0.013 mM (79%), at 25°C in 50 mM potassium phosphate (pH 6.5)	Gluconobacter oxydans	cyanide
0.013	-	at 25°C in 50 mM potassium phosphate (pH 6.5)	Gluconobacter oxydans	2-Heptyl-4-hydroxyquinoline-N-oxide
0.013	-	at 25°C in 50 mM potassium phosphate (pH 6.5)	Gluconobacter oxydans	LL-Z1272gamma
0.017	-	at 25°C in 50 mM potassium phosphate (pH 6.5)	Gluconobacter oxydans	antimycin A
0.04	-	at 25°C in 50 mM potassium phosphate (pH 6.5)	Gluconobacter oxydans	Gramicidin S
13	-	quinol oxidase activity of the membranes from the wild type shows a biphasic dependence on the cyanide concentration with the IC50 of 0.008 mM (relative amplitude, 21%) and 13 mM (79%), at 25°C in 50 mM potassium phosphate (pH 6.5)	Gluconobacter oxydans	cyanide

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Release 2023.1 (January 2023)