Literature summary for 1.10.3.1 extracted from

Virador, V.M.; Reyes Grajeda, J.P.; Blanco-Labra, A.; Mendiola-Olaya, E.; Smith, G.M.; Moreno, A.; Whitaker, J.R.
Cloning, sequencing, purification, and crystal structure of Grenache (Vitis vinifera) polyphenol oxidase (2010), J. Agric. Food Chem., 58, 1189-1201.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 30% (w/v) PEG-4000 in 100 mM buffer citrate pH 5.6 and 200 mM ammonium acetate, at 18°C	Vitis vinifera

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40000	-	SDS-PAGE	Vitis vinifera
56700	-	calculated from amino acid sequence	Vitis vinifera

Organism

Organism	UniProt	Comment	Textmining
Vitis vinifera	P93622	cultivar Grenache	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, Superose-R 12 column chromatography, and Sephadex G-75 gel filtration	Vitis vinifera

Source Tissue

Source Tissue	Comment	Organism	Textmining
fruit	-	Vitis vinifera	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 catechol + O2	-	Vitis vinifera	2 1,2-benzoquinone + 2 H2O	-	?
4-tert-butylcatechol + O2	-	Vitis vinifera	4-tert-butyl-1,2-benzoquinone + H2O	-	?
additional information	tyrosine is not a substrate	Vitis vinifera	?	-	?

Synonyms

Synonyms	Comment	Organism
1,2-benzenediol:oxygen oxidoreductase	-	Vitis vinifera
catecholase	-	Vitis vinifera
polyphenol oxidase	-	Vitis vinifera
PPO	-	Vitis vinifera

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Release 2023.1 (January 2023)